unc-94 Tropomodulin [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: unc-94
Official Full Name: Tropomodulin
Primary source: WormBase:WBGene00006823
Locus tag: CELE_C06A5.7
See related: AllianceGenome:WB:WBGene00006823
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable tropomyosin binding activity. Involved in several processes, including myofibril assembly; nematode larval development; and pointed-end actin filament capping. Located in striated muscle thin filament and terminal web. Is expressed in several structures, including intestine; non-striated muscle; pharynx; reproductive system; and seam cell. Human ortholog(s) of this gene implicated in dilated cardiomyopathy 2G; megacystis-microcolon-intestinal hypoperistalsis syndrome; and nemaline myopathy 10. Orthologous to several human genes including TMOD1 (tropomodulin 1); TMOD2 (tropomodulin 2); and TMOD3 (tropomodulin 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See unc-94 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 12

Sequence:: Chromosome: I; NC_003279.8 (5992661..6003379, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The data support a model in which minus-end actin capping by UNC-94 promotes proper F-actin structure and contraction in the terminal web, yielding proper shape of the intestinal lumen.
Title: The minus-end actin capping protein, UNC-94/tropomodulin, regulates development of the Caenorhabditis elegans intestine.
In vitro, UNC-94 acts in combination with HMP-1, leading to longer actin bundles than with HMP-1 alone.
Title: Tropomodulin protects α-catenin-dependent junctional-actin networks under stress during epithelial morphogenesis.
study shows that the mutationally identified gene unc-94 corresponds with the sequence-predicted gene tmd-1 (C06A5.7), which encodes a tropomodulin
Title: unc-94 encodes a tropomodulin in Caenorhabditis elegans.
C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans was expressed in Escherichia coli and purified to homogeneity
Title: Purification, nanocrystallization and preliminary X-ray analysis of a C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans.
cTmd1 consists of AAs 196-392. The chain is well defined in the 2Fo-Fc map for the range 222-388. Much of the N-terminus, including AAs 196-221, is entirely disordered. The C-terminus of cTmd1 has 6 additional AAs. 4 of them (389-392) are disordered.
Title: Structural genomics of Caenorhabditis elegans: crystal structure of the tropomodulin C-terminal domain.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C06A5.7 (e-PCR)
- UniSTS:313482

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IC Inferred by Curator more info	PubMed
enables tropomyosin binding	IBA Inferred from Biological aspect of Ancestor more info
enables tropomyosin binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in muscle thin filament assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in muscle thin filament assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in myofibril assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in nematode larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pointed-end actin filament capping	IDA Inferred from Direct Assay more info	PubMed
involved_in pointed-end actin filament capping	IEA Inferred from Electronic Annotation more info
involved_in sarcomere organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in myofibril	IBA Inferred from Biological aspect of Ancestor more info
located_in striated muscle thin filament	IDA Inferred from Direct Assay more info	PubMed
located_in terminal web	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Tropomodulin

NP_001379540.1

Confirmed by transcript evidence

NP_491735.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

5992661..6003379 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001392388.1 → NP_001379540.1 Tropomodulin [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/Swiss-Prot

O01479, Q95Q88

Conserved Domains (2) summary

pfam03250
Location:33 → 182

Tropomodulin

cl39015
Location:236 → 386

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...
NM_059334.5 → NP_491735.1 Tropomodulin [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_491735.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O01479

Conserved Domains (2) summary

cd00116
Location:245 → 395

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

pfam03250
Location:45 → 191

Tropomodulin; Tropomodulin