Nkx6-1 NK6 homeobox 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nkx6-1provided by RGD
Official Full Name: NK6 homeobox 1provided by RGD
Primary source: RGD:69318
See related: EnsemblRapid:ENSRNOG00000002149 AllianceGenome:RGD:69318
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Nkx61; Nkx6a; Nkx6.1
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Involved in several processes, including positive regulation of insulin secretion; type B pancreatic cell development; and type B pancreatic cell proliferation. Predicted to be active in nucleus. Orthologous to human NKX6-1 (NK6 homeobox 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 1.8), Brain (RPKM 0.6) and 4 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 14p22

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	14	NC_086032.1 (8274238..8281171)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	14	NC_051349.1 (7969756..7976689)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	14	NC_005113.4 (9555264..9562197)

Chromosome 14 - NC_086032.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deficiency of transcription factor Nkx6.1 does not prevent insulin secretion in INS-1E cells.
Title: Deficiency of transcription factor Nkx6.1 does not prevent insulin secretion in INS-1E cells.
The beta-cell maintenance factor Nkx6.1 mRNA and protein were declining with age (>12-fold, 10 months) and decreasing with fasting hyperglycemia in Goto Kakizaki rats, probably predetermining the impaired mtDNA replication (copy number decrease), while spatial expansion of mtDNA kept nucleoids with only smaller sizes than those containing much higher mtDNA in non-diabetic beta-cells.
Title: Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle nucleoid size decrease in pancreatic islet β-cells of diabetic Goto Kakizaki rats.
These data demonstrate that c-Fos is critical for Nkx6.1-mediated expansion of functional beta-cell mass.
Title: Nkx6.1-mediated insulin secretion and β-cell proliferation is dependent on upregulation of c-Fos.
The homeobox beta-cell transcription factor Nkx6.1 induces beta-cell proliferation by activating the orphan nuclear receptors Nr4a1 and Nr4a3.
Title: Aurora Kinase A is critical for the Nkx6.1 mediated β-cell proliferation pathway.
Nkx6.1 plays a vital role in astrocyte specification and differentiation in the ventral spinal cord.
Title: Control of astrocyte progenitor specification, migration and maturation by Nkx6.1 homeodomain transcription factor.
Nkx6.1 and Nr4a receptors induce components of the anaphase-promoting complex, including ubiquitin-conjugating enzyme E2C, resulting in degradation of the cell cycle inhibitor p21
Title: Nkx6.1 regulates islet β-cell proliferation via Nr4a1 and Nr4a3 nuclear receptors.
K cells could be reprogrammed partially to beta-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture.
Title: Reprogramming of enteroendocrine K cells to pancreatic β-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture.
Overexpression of Nkx6.1 in islets caused a clear enhancement of glucose-stimulated insulin secretion.
Title: Stimulation of human and rat islet beta-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1.
Nkx6.1 homeodomain transcription factor suppresses glucagon expression and regulates glucose-stimulated insulin secretion in islet beta cells.
Title: The Nkx6.1 homeodomain transcription factor suppresses glucagon expression and regulates glucose-stimulated insulin secretion in islet beta cells.
Expression in pancreatic beta cells is enhanced by peroxisome proliferator-activated receptor-gamma.
Title: Peroxisome proliferator-activated receptor-gamma regulates expression of PDX-1 and NKX6.1 in INS-1 cells.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

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Markers

Nkx6-1 (e-PCR), detects polymorphism
- UniSTS:516530

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to cytokine stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to peptide hormone stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within central nervous system neuron differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within endocrine pancreas development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of oligodendrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of neuron differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of oligodendrocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within oligodendrocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pancreatic A cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of neuron differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of oligodendrocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of axon extension	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of neuron migration	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in response to nicotine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within smoothened signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in type B pancreatic cell development	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within type B pancreatic cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in type B pancreatic cell proliferation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: homeobox protein Nkx-6.1

Names: NK homeobox, family 6, A; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1; homeobox protein NK-6 homolog A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.