CBK1 serine/threonine protein kinase CBK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CBK1
Official Full Name: serine/threonine protein kinase CBK1
Primary source: SGD:S000005105
Locus tag: YNL161W
See related: AllianceGenome:SGD:S000005105; FungiDB:YNL161W; VEuPathDB:YNL161W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including budding cell apical bud growth; establishment or maintenance of actin cytoskeleton polarity; and regulation of fungal-type cell wall organization. Located in several cellular components, including cellular bud tip; incipient cellular bud site; and mating projection tip. Orthologous to several human genes including STK38 (serine/threonine kinase 38). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CBK1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (332597..334867)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fir1 is an intrinsically disordered protein localized to the cytokinesis site that is degraded upon septum completion but stabilized when septation is aberrant. Fir1 protects cytokinesis in part by inhibiting a separation-specific exocytosis function of the Cbk1, a key component of "hippo" signaling that induces mother-daughter separation.
Title: A cell separation checkpoint that enforces the proper order of late cytokinetic events.
Cbk1-Mob2 complex shows a novel coactivator-organized activation region that may be unique to NDR/LATS kinases, in which a key regulatory motif apparently shifts from an inactive binding mode to an active one upon phosphorylation
Title: The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism.
Cbk1 and Bck2 are required for Mpk1 activation during heat shock and cell wall stress and for Mpk1 dephosphorylation during heat shock adaptation.
Title: Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock.
propose that Cdc14 coordinates cell separation with mitotic exit via FEAR-initiated phosphorylation of the Cbk1 HM site and MEN-activated reversal of mitotic CDK phosphorylations that block both Cbk1 and Ace2 function
Title: Mitotic exit control of the Saccharomyces cerevisiae Ndr/LATS kinase Cbk1 regulates daughter cell separation after cytokinesis.
Data show that Ace2's nuclear localization is maintained by continuous Cbk1 activity and that inhibition of the kinase leads to immediate loss of phosphorylation and export to the cytoplasm.
Title: Sequential counteracting kinases restrict an asymmetric gene expression program to early G1.
Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control.
Title: Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control.
Mutations in BRR1 or MPT5 lead to a restoration of fertility induced by cbk1p mutation, and a more-or-less pronounced restoration of polarity; they also show genetic interactions with SSD1.
Title: Mutations in the Saccharomyces cerevisiae kinase Cbk1p lead to a fertility defect that can be suppressed by the absence of Brr1p or Mpt5p (Puf5p), proteins involved in RNA metabolism.
CBK1-S745F allele leads to a constitutively active form of Cbk1p that is independent of the upstream RAM network.
Title: Mutations in the C-terminus of the conserved NDR kinase, Cbk1p of Saccharomyces cerevisiae, make the protein independent of upstream activators.
These data suggest that an important function for Cbk1 and the RAM signaling network is to regulate growth and secretion via Golgi and Sec2/Sec4-dependent processes.
Title: The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion.
Cbk1 exhibits a novel specificity that is likely conserved among related kinases from yeast to metazoans. Cbk1 is functionally restricted to the daughter cell, and cannot diffuse from the daughter to the mother.
Title: The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in budding cell apical bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in budding cell apical bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in establishment or maintenance of actin cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of fungal-type cell wall organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septum digestion after cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	HDA more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in prospore membrane	HDA more info	PubMed

General protein information

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Preferred Names: serine/threonine protein kinase CBK1

NP_014238.3

EC 2.7.11.1

Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

332597..334867

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182999.3 → NP_014238.3 TPA: serine/threonine protein kinase CBK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014238.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W122, P53894

UniProtKB/TrEMBL

A6ZRS3, G2WLU6, N1NXC7

Conserved Domains (1) summary

cd05629
Location:350 → 748

STKc_NDR_like_fungal; Catalytic domain of Fungal Nuclear Dbf2-Related kinase-like Serine/Threonine Kinases