robo2 roundabout 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: robo2provided by FlyBase
Official Full Name: roundabout 2provided by FlyBase
Primary source: FLYBASE:FBgn0002543
Locus tag: Dmel_CG5481
See related: AllianceGenome:FB:FBgn0002543
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-EST:Liang-1.75; CG14347; CG14348; CG5481; CG5574; clone 1.75; CT17326; D-robo2; D-Robo2; Dmel\CG5481; dRobo-2; fus4; lea; robo; Robo; Robo 2; robo-2; Robo-2; Robo2; ROBO2; Robo2 (Lea)
Summary: Enables protein homodimerization activity. Involved in several processes, including heart development; nervous system development; and regulation of cell migration. Located in axon. Is expressed in several structures, including cardiac outflow muscle cell; embryonic thoracic segment; epidermis; sensory neurons; and tracheal branch primordium. Human ortholog(s) of this gene implicated in autosomal recessive congenital nystagmus; congenital nystagmus; multiple myeloma; panhypopituitarism; and plasmacytoma. Orthologous to human ROBO1 (roundabout guidance receptor 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 22A1-22A1; 2-3 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (1380086..1420453, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (1380086..1420453, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Characterization of enhancer fragments in Drosophila robo2.
Title: Characterization of enhancer fragments in Drosophila robo2.
The Slit-binding Ig1 domain is required for multiple axon guidance activities of Drosophila Robo2.
Title: The Slit-binding Ig1 domain is required for multiple axon guidance activities of Drosophila Robo2.
repulsive Slit-Robo2-Ena signaling exerts an extrusive force downstream of JNK to eliminate scrib cells from epithelia by disrupting E-cadherin.
Title: Slit-Robo Repulsive Signaling Extrudes Tumorigenic Cells from Epithelia.
The authors describe a distinct mechanism to inhibit Robo1 repulsion and promote midline crossing, in which Roundabout2 (Robo2) binds to and prevents Robo1 signaling.
Title: Robo2 acts in trans to inhibit Slit-Robo1 repulsion in pre-crossing commissural axons.
a Robo2-mediated mechanism, independent of signaling, is essential to limiting Slit distribution and which might be relevant to the regulation of Slit-mediated short-range signaling in additional systems
Title: A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning.
Data identify Robo2 and Robo3 as key effectors of Hb9 in regulating nervous system development.
Title: The homeodomain transcription factor Hb9 controls axon guidance in Drosophila through the regulation of Robo receptors.
Data show that Robo and Robo2 promote midline repulsion, while Robo2 and Robo3 specify the position of longitudinal axon pathways.
Title: Slit/Robo-mediated axon guidance in Tribolium and Drosophila: divergent genetic programs build insect nervous systems.
Study found that lateral positioning does not rely on structural differences between the Robo1 and Robo2 receptors-neither in their ectodomain nor cytoplasmic domains instead expression differences alone can account for lateral pathway selection.
Title: Distinct protein domains and expression patterns confer divergent axon guidance functions for Drosophila Robo receptors.
Results describe the role of Slit and its transmembrane receptors Roundabout (Robo) and Roundabout2 (Robo2) during morphogenesis of the Drosophila heart tube, a process analogous to early heart formation in vertebrates.
Title: Lateral positioning at the dorsal midline: Slit and Roundabout receptors guide Drosophila heart cell migration.
robo2 is required for heart assembly
Title: Slit coordinates cardiac morphogenesis in Drosophila.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables axon guidance receptor activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Roundabout signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in axon guidance	IEA Inferred from Electronic Annotation more info
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon midline choice point recognition	IEA Inferred from Electronic Annotation more info
involved_in cardioblast cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in central complex development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic heart tube development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mushroom body development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in outflow tract morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of epithelial cell migration, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in salivary gland boundary specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic target inhibition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in synaptic target recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral cord development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: roundabout 2

Names: CG5481-PA; CG5481-PB; Robo2; Roundabout2; SGP cluster fusion defects 4; leak; robo; robo2-PA; robo2-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

1380086..1420453 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080531.4 → NP_536792.2 roundabout 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_536792.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9GPP7, Q9VQ08

Related

FBpp0077606

Conserved Domains (7) summary

cd00096
Location:496 → 561

Ig; Immunoglobulin domain

cd05724
Location:193 → 279

Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:90 → 185

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

cd00063
Location:588 → 681

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:283 → 370

I-set; Immunoglobulin I-set domain

pfam00041
Location:864 → 952

fn3; Fibronectin type III domain

cl11960
Location:300 → 370

Ig; Immunoglobulin domain
NM_001272939.2 → NP_001259868.1 roundabout 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259868.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PC46, Q9GPP7

Conserved Domains (7) summary

cd00096
Location:496 → 561

Ig; Immunoglobulin domain

cd05724
Location:193 → 279

Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:90 → 185

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

cd00063
Location:588 → 681

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:283 → 370

I-set; Immunoglobulin I-set domain

pfam00041
Location:864 → 952

fn3; Fibronectin type III domain

cl11960
Location:300 → 370

Ig; Immunoglobulin domain