YPK1 serine/threonine protein kinase YPK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YPK1
Official Full Name: serine/threonine protein kinase YPK1
Primary source: SGD:S000001609
Locus tag: YKL126W
See related: AllianceGenome:SGD:S000001609; FungiDB:YKL126W; VEuPathDB:YKL126W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SLI2
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including cellular response to acetate; eisosome assembly; and regulation of fungal-type cell wall organization. Located in cellular bud neck; cytosol; and plasma membrane. Human ortholog(s) of this gene implicated in hypertension. Orthologous to several human genes including C8orf44-SGK3 (C8orf44-SGK3 readthrough); SGK1 (serum/glucocorticoid regulated kinase 1); and SGK2 (serum/glucocorticoid regulated kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See YPK1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (205707..207749)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of sphingolipid biosynthesis in the endoplasmic reticulum via signals from the plasma membrane in budding yeast.
Title: Regulation of sphingolipid biosynthesis in the endoplasmic reticulum via signals from the plasma membrane in budding yeast.
TORC2-Dependent Ypk1-Mediated Phosphorylation of Lam2/Ltc4 Disrupts Its Association with the beta-Propeller Protein Laf1 at Endoplasmic Reticulum-Plasma Membrane Contact Sites in the Yeast Saccharomyces cerevisiae.
Title: TORC2-Dependent Ypk1-Mediated Phosphorylation of Lam2/Ltc4 Disrupts Its Association with the β-Propeller Protein Laf1 at Endoplasmic Reticulum-Plasma Membrane Contact Sites in the Yeast Saccharomyces cerevisiae.
Ypk1 activity requires stimulation by TORC2-mediated phosphorylation at Ser644 and Thr662.
Title: The Stress-Sensing TORC2 Complex Activates Yeast AGC-Family Protein Kinase Ypk1 at Multiple Novel Sites.
We identified Ypk signaling as a pathway likely to be activated under these conditions, and we identified potential Ypk1 target proteins. Our data provide a rich resource for on-going mechanistic studies of key elements of the cellular response to the depletion of sphingolipid levels and the maintenance of sphingolipid homeostasis
Title: Proteomic and phosphoproteomic analyses of yeast reveal the global cellular response to sphingolipid depletion.
Data indicate that mutant nce102 sng1 cells show features of impaired Pkh/Ypk signalling, including increased reactive oxygen species accumulation.
Title: Sng1 associates with Nce102 to regulate the yeast Pkh-Ypk signalling module in response to sphingolipid status.
Moreover, hyperosmotic conditions block TORC2-dependent Ypk1-mediated Fps1 phosphorylation, causing channel closure, glycerol accumulation, and enhanced survival under hyperosmotic stress.
Title: Down-regulation of TORC2-Ypk1 signaling promotes MAPK-independent survival under hyperosmotic stress.
Data indicate that links between TOR complex 2 (TORC2)/kinase Ypk1 signaling and reactive oxygen species (ROS) as regulators of actin cytoskeleton.
Title: TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species.
Ypk1 and Plb1 interact to alter sphingolipid metabolism up-regulates PLB1 expression via Crz1
Title: Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation.
findings both extend the scope of cellular processes regulated by Ypk1/2 to include negative regulation of calcineurin and broaden the repertoire of mechanisms for membrane recruitment and activation of a protein kinase
Title: Plasma membrane recruitment and activation of the AGC kinase Ypk1 is mediated by target of rapamycin complex 2 (TORC2) and its effector proteins Slm1 and Slm2.
Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae.
Title: Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell wall organization	IEA Inferred from Electronic Annotation more info
involved_in cellular response to acetate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in eisosome assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in glycerophospholipid metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glycerophospholipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of phospholipid translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of sphingolipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of fungal-type cell wall organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of fungal-type cell wall organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine protein kinase YPK1

NP_012796.1

EC 2.7.11.1

S/T protein kinase; required for PM lipid and protein homeostasis; involved in sphingolipid-mediated and cell integrity signaling pathways and endocytosis; phosphorylates and downregulates Fpk1p; phosphorylates and inactivates Orm1p/2p when sphingolipid synthesis is compromised; role in TORC1/2-dependent Rps6a/b phosphorylation; phosphorylated and activated by TORC2 and by Pkh1/2p in the presence of phytosphingosine; human homolog SGK1 complements the null and inviability of a ypk1 ypk2 mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

205707..207749

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179692.1 → NP_012796.1 TPA: serine/threonine protein kinase YPK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012796.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VX69, P12688

UniProtKB/TrEMBL

A6ZZJ4, B3LQX3, B5VM35, C8ZC49, G2WHR1, N1P163

Conserved Domains (2) summary

cd11651
Location:116 → 342

YPK1_N_like; Fungal protein kinase domain similar to the N-terminus of YPK1

cl21453
Location:352 → 663

PKc_like; Protein Kinases, catalytic domain