MAD1 coiled-coil domain-containing protein MAD1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MAD1
Official Full Name: coiled-coil domain-containing protein MAD1
Primary source: SGD:S000003054
Locus tag: YGL086W
See related: AllianceGenome:SGD:S000003054; FungiDB:YGL086W; VEuPathDB:YGL086W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in negative regulation of protein import into nucleus during spindle assembly checkpoint; nucleocytoplasmic transport; and regulation of cell cycle phase transition. Located in kinetochore and nucleus. Human ortholog(s) of this gene implicated in lymphoma; mosaic variegated aneuploidy syndrome 7 with inflammation and tumor predisposition; and prostate cancer. Orthologous to human MAD1L1 (mitotic arrest deficient 1 like 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MAD1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (347119..349368)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mad1 recruitment to the kinetochore and it's role in the spindle assembly checkpoint
Title: Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.
Mad1 is recruited by detached kinetochores competing with alternate other sites in nucleus: Mad1 pool is small enough to be fully depleted by one pair of detached kinetochores. Lateral attachment licenses Mad1 removal from kinetochores after kinetic delay
Title: Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment.
Mad1p senses unattached kinetochores and, by altering nuclear pore complex transport activity, regulates the nuclear environment of the spindle.
Title: Mitosis-specific regulation of nuclear transport by the spindle assembly checkpoint protein Mad1p.
cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension.
Title: Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis.
Only the C terminus is required for spindle assembly checkpoint (SAC) function and the nuclear pore complex, through Nup53p, may act to regulate the duration of the SAC response.
Title: Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiae.
Saccharomyces cerevisiae Mad1p C-terminal domain mediates spindle checkpoint function, chromosome transmission fidelity and CEN association
Title: The C-terminal half of Saccharomyces cerevisiae Mad1p mediates spindle checkpoint function, chromosome transmission fidelity and CEN association.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in activation of mitotic cell cycle spindle assembly checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in attachment of mitotic spindle microtubules to kinetochore	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic DNA integrity checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein import into nucleus during spindle assembly checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleocytoplasmic transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleocytoplasmic transport	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in mitotic spindle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: coiled-coil domain-containing protein MAD1

NP_011429.3

Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

347119..349368

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180951.3 → NP_011429.3 TPA: coiled-coil domain-containing protein MAD1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011429.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU58, P40957

UniProtKB/TrEMBL

B3LHF2, N1P9Q2

Conserved Domains (1) summary

pfam05557
Location:83 → 747

MAD; Mitotic checkpoint protein