Diap2 Death-associated inhibitor of apoptosis 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Diap2provided by FlyBase
Official Full Name: Death-associated inhibitor of apoptosis 2provided by FlyBase
Primary source: FLYBASE:FBgn0015247
Locus tag: Dmel_CG8293
See related: AllianceGenome:FB:FBgn0015247
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG8293; D-iap2; D-IAP2; dIAP; Diap; DIAP; Diap-2; DIAP-2; DIAP1; diap2; dIAP2; DIAP2; DIAPII; Diha; DIHA; dILP; Dmel\CG8293; IAP; Iap2; IAP2; Ilp
Summary: Enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process; enzyme binding activity; and ubiquitin protein ligase activity. Involved in several processes, including defense response to bacterium; positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria; and protein polyubiquitination. Located in perinuclear region of cytoplasm. Is expressed in Malpighian tubule and organism. Human ortholog(s) of this gene implicated in X-linked lymphoproliferative syndrome 2; breast cancer; carcinoma (multiple); extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue; and prostate cancer. Orthologous to several human genes including BIRC2 (baculoviral IAP repeat containing 2) and BIRC3 (baculoviral IAP repeat containing 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 52D2-52D2; 2-76 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (15932170..15934825, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (11819675..11822330, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Drice restrains Diap2-mediated inflammatory signalling and intestinal inflammation.
Title: Drice restrains Diap2-mediated inflammatory signalling and intestinal inflammation.
This suggests that DIAP2 and LUBEL work together to promote Kenny-mediated activation of Relish..We found LUBEL-mediated M1-Ub chain formation to be required for flies to survive oral infection with Gram-negative bacteria, for activation of Relish-mediated expression of antimicrobial peptide genes and for pathogen clearance during oral infection
Title: M1-linked ubiquitination by LUBEL is required for inflammatory responses to oral infection in Drosophila.
cleaved protein retains the ability to interact with immune deficiency pathway signal transduction
Title: Independent Proteolytic Activities Control the Stability and Size of Drosophila Inhibitor of Apoptosis 2 Protein.
The authors show that stimulation of the Drosophila pattern recognition protein PGRP-LCx induces DIAP2-dependent polyubiquitylation of the initiator caspase DREDD.
Title: Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling.
Because DIAP2 efficiently interacts with drICE, our data suggest that DIAP2 controls drICE in its apoptotic and nonapoptotic roles.
Title: DIAP2 functions as a mechanism-based regulator of drICE that contributes to the caspase activity threshold in living cells.
Required to sustain antimicrobial peptide gene expression; inactivation results in impaired microbial resistance.
Title: Iap2 is required for a sustained response in the Drosophila Imd pathway.
role in inhibiting DrICE
Title: Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid and Grim.
Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Imd pathway, which controls antimicrobial peptide expression in Drosophila.
Title: Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway.
DIAP1 ensures cell viability by directly inhibiting caspases.
Title: IAP-antagonists exhibit non-redundant modes of action through differential DIAP1 binding.
precise timing of larval tissue cell death during metamorphosis is achieved through a steroid-triggered shift in the balance between the Drosophila inhibitor of apoptosis 1 and the rpr and hid death activators
Title: A balance between the diap1 death inhibitor and reaper and hid death inducers controls steroid-triggered cell death in Drosophila.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables caspase binding	IPI Inferred from Physical Interaction more info	PubMed
enables cysteine-type endopeptidase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type endopeptidase regulator activity involved in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	ISM Inferred from Sequence Model more info	PubMed

Process	Evidence Code	Pubs
involved_in chaeta development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidoglycan recognition protein signaling pathway	HMP more info	PubMed
involved_in peptidoglycan recognition protein signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidoglycan recognition protein signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria	HMP more info	PubMed
involved_in positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Death-associated inhibitor of apoptosis 2

Names: CG8293-PA; CG8293-PB; Diap2-PA; Diap2-PB; IAP-like protein; drosophila inhibitor of apoptosis 2; drosophila melanogaster inhibitor of apoptosis 2; inhibitor of apoptosis 2; inhibitor of apoptosis protein 2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

15932170..15934825 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_176182.2 → NP_788362.1 Death-associated inhibitor of apoptosis 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788362.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZI4, Q24115, Q24149, Q24177, Q24307, Q960U3, Q9V7G1

Related

FBpp0086432

Conserved Domains (4) summary

smart00238
Location:112 → 181

BIR; Baculoviral inhibition of apoptosis protein repeat

cd00022
Location:213 → 281

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

cd14321
Location:320 → 363

UBA_IAPs; UBA domain found in inhibitor of apoptosis proteins (IAPs)

pfam13920
Location:447 → 492

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)
NM_057779.5 → NP_477127.1 Death-associated inhibitor of apoptosis 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477127.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZI4, Q24115, Q24149, Q24177, Q24307, Q960U3, Q9V7G1

Related

FBpp0086431

Conserved Domains (4) summary

smart00238
Location:112 → 181

BIR; Baculoviral inhibition of apoptosis protein repeat

cd00022
Location:213 → 281

BIR; Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. ...

cd14321
Location:320 → 363

UBA_IAPs; UBA domain found in inhibitor of apoptosis proteins (IAPs)

pfam13920
Location:447 → 492

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)