DNA2 bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DNA2
Official Full Name: bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2
Primary source: SGD:S000001207
Locus tag: YHR164C
See related: AllianceGenome:SGD:S000001207; FungiDB:YHR164C; VEuPathDB:YHR164C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: WEB2
Summary: Enables DNA binding activity; catalytic activity, acting on DNA; and protein serine/threonine kinase activator activity. Involved in DNA metabolic process; G-quadruplex DNA unwinding; and mitotic DNA replication checkpoint signaling. Located in chromosome, telomeric region; cytoplasm; and nucleus. Used to study Bloom syndrome; Seckel syndrome (multiple); Werner syndrome; cancer; and microcephaly. Human ortholog(s) of this gene implicated in Seckel syndrome 8; autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 6; isolated growth hormone deficiency type IA; and mitochondrial myopathy. Orthologous to human DNA2 (DNA replication helicase/nuclease 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DNA2 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (424610..429178, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of Dna2 fidelity results in decreased Exo1-mediated resection at DNA double-strand breaks.
Title: Loss of Dna2 fidelity results in decreased Exo1-mediated resection at DNA double-strand breaks.
Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.
Title: Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.
The secondary-structured DNA-binding activity of Dna2 endonuclease/helicase is critical to cell growth under replication stress.
Title: The secondary-structured DNA-binding activity of Dna2 endonuclease/helicase is critical to cell growth under replication stress.
in addition to Dna2 phosphorylation described previously, Dna2 sumoylation is required for the homeostasis of the Dna2 protein function to promote genome stability
Title: Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2.
By conditionally depleting lagging-strand nucleases and directly analyzing Okazaki fragments synthesized in vivo in Saccharomyces cerevisiae, we conduct a systematic evaluation of the impact of Rad27, Dna2 and Exo1 on lagging-strand synthesis. We find that Rad27 processes the majority of lagging-strand flaps, with a significant additional contribution from Exo1 but not from Dna2.
Title: Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo.
presentation of a model in which alternative processing of DNA structures arising in Dna2-deficient cells can result in the release of DNA fragments and their capture at double strand breaks (DSBs); similar DNA insertions at DSBs are expected to occur in any cells with linear extrachromosomal DNA fragments
Title: Dna2 nuclease deficiency results in large and complex DNA insertions at chromosomal breaks.
DNA nick created by MRX-Sae2 at Ku-blocked DNA double-strand breaks ends can serve as the entry site for Exo1 or Sgs1-Dna2.
Title: A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1-Dna2 in long-range DNA end resection.
Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement.
Title: Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement.
Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1.
Title: Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1.
role of the Dna2 translocase activity in DNA break end resection and in the imposition of the 5' strand specificity of end resection.
Title: A novel role of the Dna2 translocase function in DNA break resection.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables 5'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-flap endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA hairpin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA hairpin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables telomeric G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G-quadruplex DNA unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in lagging strand elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic DNA replication checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in replication fork reversal	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2

NP_012034.1

EC 3.6.4.12

ATP-dependent 5'-flap endonuclease and DNA helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; cell cycle-dependent localization to telomeric chromatin and replicating chromosomes; required for telomerase-dependent telomere synthesis; stability, localization and nuclease activity regulated by sumoylation; forms nuclear foci upon replication stress; human homolog DNA2 complements yeast dna2 mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

424610..429178 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179295.1 → NP_012034.1 TPA: bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012034.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLB3, P38859

UniProtKB/TrEMBL

A6ZT72, C7GJX6, G2WFN8, N1P9H3

Conserved Domains (2) summary

COG1112
Location:551 → 1481

DNA2; Superfamily I DNA and/or RNA helicase [Replication, recombination and repair]

cd18041
Location:1052 → 1253

DEXXQc_DNA2; DEXXQ-box helicase domain of DNA2