Ppp1r12a protein phosphatase 1, regulatory subunit 12A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ppp1r12aprovided by RGD
Official Full Name: protein phosphatase 1, regulatory subunit 12Aprovided by RGD
Primary source: RGD:620013
See related: EnsemblRapid:ENSRNOG00000004925 AllianceGenome:RGD:620013
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: M110; MBSP; Mypt1
Summary: Enables phosphoprotein phosphatase activity. Predicted to be involved in several processes, including centrosome cycle; positive regulation of transcription by RNA polymerase II; and protein dephosphorylation. Predicted to act upstream of or within cellular response to xenobiotic stimulus and regulation of nucleocytoplasmic transport. Predicted to be located in several cellular components, including contractile muscle fiber; cytoskeleton; and kinetochore. Predicted to be part of PTW/PP1 phosphatase complex. Predicted to be active in A band and Z disc. Orthologous to human PPP1R12A (protein phosphatase 1 regulatory subunit 12A). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Uterus (RPKM 270.9), Heart (RPKM 247.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q21

Exon count:: 27

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (45368922..45480158)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (43482808..43593689)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (51404971..51515382, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

reduction of blood pressure elevation by losartan in spontaneously hypertensive rats occurs through the suppression of LARG expression and MYPT-1 phosphorylation in vascular smooth muscle cells
Title: Reduction of blood pressure elevation by losartan in spontaneously hypertensive rats through suppression of LARG expression in vascular smooth muscle cells.
Phos-tag SDS-PAGE thus provides a suitable and convenient method for analysis of the complex, multisite MYPT1 phosphorylation events involved in the regulation of myosin light chain phosphatase activity and smooth muscle contraction.
Title: Analysis of phosphorylation of the myosin-targeting subunit of myosin light chain phosphatase by Phos-tag SDS-PAGE.
During cGMP-mediated signaling, aa 888-928 of MYPT1 mediate the PKGIalpha-MYPT1 interaction.
Title: MYPT1 mutants demonstrate the importance of aa 888-928 for the interaction with PKGIalpha.
Both atorvastatin (10(-4) m) and Y-27632 (10(-6) m) pretreatment inhibited the phosphorylation of myosin phosphatase target subunit-1 (MYPT-1).
Title: Atorvastatin acutely reduces the reactivity to spasmogens in rat aorta: implication of the inhibition of geranylgeranylation and MYPT-1 phosphorylation.
EGF induces Ca(2+) sensitization in vascular smooth muscle by Rho-kinase-dependent inactivation of MLCP mediated by the EGF receptor/MEK/Erk1/2 pathway
Title: Epidermal growth factor induces Ca(2+) sensitization through Rho-kinase-dependent phosphorylation of myosin phosphatase target subunit 1 in vascular smooth muscle.
nuclear translocation of CaMKIIdelta3 following dephosphorylation at Ser(332) by PP1 likely accounts for BDNF expression and subsequent neurite extension and survival of dopaminergic neurons.
Title: Nuclear Translocation of Calcium/Calmodulin-dependent Protein Kinase IIδ3 Promoted by Protein Phosphatase-1 Enhances Brain-derived Neurotrophic Factor Expression in Dopaminergic Neurons.
These results indicate that PPP1R12A indeed plays a role in skeletal muscle insulin signaling
Title: Quantitative phosphoproteomics reveals novel phosphorylation events in insulin signaling regulated by protein phosphatase 1 regulatory subunit 12A.
Rac1 coordinates with RhoA to increase myosin light chain (MLC) phosphorylation by inactivation of CPI-17/MYPT1 phosphatase, which decreases MLC phosphatase activity thus promoting MLC phosphorylation and cell contraction
Title: Rac1 regulates myosin II phosphorylation through regulation of myosin light chain phosphatase.
During acute myocardial ischemia MYPT1 is targeted for degradation or proteolysis. Reduced MYPT1 expression results in a decrease in both tone and the sensitivity to NO-mediated SM relaxation of the peripheral vasculature.
Title: Altered reactivity of tertiary mesenteric arteries following acute myocardial ischemia.
decreased insulin-stimulated MRIP/MYPT1 association may contribute to the impaired insulin-mediated vasodilation observed in the diabetic vasculature
Title: Impaired insulin-stimulated myosin phosphatase Rho-interacting protein signaling in diabetic Goto-Kakizaki vascular smooth muscle cells.

Submit: New GeneRIF Correction See all GeneRIFs (28)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH137913 (e-PCR)
- UniSTS:220000

RH131122 (e-PCR)
- UniSTS:214405

AU047441 (e-PCR)
- UniSTS:110506

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	IEA Inferred from Electronic Annotation more info
enables 14-3-3 protein binding	ISO Inferred from Sequence Orthology more info
enables 14-3-3 protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables enzyme inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables enzyme inhibitor activity	IEA Inferred from Electronic Annotation more info
enables enzyme inhibitor activity	ISO Inferred from Sequence Orthology more info
enables enzyme inhibitor activity	ISS Inferred from Sequence or Structural Similarity more info
enables myosin phosphatase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatase regulator activity	IEA Inferred from Electronic Annotation more info
enables phosphatase regulator activity	ISO Inferred from Sequence Orthology more info
enables phosphatase regulator activity	ISS Inferred from Sequence or Structural Similarity more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
involved_in centrosome cycle	IEA Inferred from Electronic Annotation more info
involved_in centrosome cycle	ISO Inferred from Sequence Orthology more info
involved_in centrosome cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of catalytic activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of catalytic activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in neuron projection morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein dephosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell adhesion	ISS Inferred from Sequence or Structural Similarity more info
NOT involved_in regulation of establishment of endothelial barrier	ISO Inferred from Sequence Orthology more info
involved_in regulation of nucleocytoplasmic transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of nucleocytoplasmic transport	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in A band	IBA Inferred from Biological aspect of Ancestor more info
located_in A band	IEA Inferred from Electronic Annotation more info
located_in A band	ISO Inferred from Sequence Orthology more info
located_in A band	ISS Inferred from Sequence or Structural Similarity more info
part_of PTW/PP1 phosphatase complex	IEA Inferred from Electronic Annotation more info
part_of PTW/PP1 phosphatase complex	ISO Inferred from Sequence Orthology more info
part_of PTW/PP1 phosphatase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in Z disc	IEA Inferred from Electronic Annotation more info
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in Z disc	ISS Inferred from Sequence or Structural Similarity more info
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in contractile muscle fiber	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in stress fiber	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein phosphatase 1 regulatory subunit 12A

Names: PP-1M; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit; protein phosphatase subunit 1M; serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053890.2 → NP_446342.2 protein phosphatase 1 regulatory subunit 12A

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

Q10728, Q62937, Q9WU33

UniProtKB/TrEMBL

A6IGD8

Related

ENSRNOP00000104388.1, ENSRNOT00000152362.1

Conserved Domains (4) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:877 → 975

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cd21944
Location:603 → 659

IPD_MYPT1; inhibitory phosphorylation domain of myosin phosphatase targeting subunit 1(MYPT1)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

45368922..45480158

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006241311.3 → XP_006241373.1 protein phosphatase 1 regulatory subunit 12A isoform X3

See identical proteins and their annotated locations for XP_006241373.1

UniProtKB/TrEMBL

A0A0G2JWJ0, A0A8I6ATJ5, A6IGD6

Related

ENSRNOP00000069900.1, ENSRNOT00000080285.3

Conserved Domains (5) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:199 → 252

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:933 → 1031

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]
XM_006241315.3 → XP_006241377.1 protein phosphatase 1 regulatory subunit 12A isoform X8

See identical proteins and their annotated locations for XP_006241377.1

UniProtKB/TrEMBL

A6IGD7, D4ACS0

Related

ENSRNOP00000040934.7, ENSRNOT00000050037.8

Conserved Domains (5) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:199 → 252

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:873 → 971

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]
XM_063262871.1 → XP_063118941.1 protein phosphatase 1 regulatory subunit 12A isoform X6
XM_063262872.1 → XP_063118942.1 protein phosphatase 1 regulatory subunit 12A isoform X10
XM_063262873.1 → XP_063118943.1 protein phosphatase 1 regulatory subunit 12A isoform X11

UniProtKB/TrEMBL

D4A1S6

Related

ENSRNOP00000045260.6, ENSRNOT00000041473.7
XM_006241308.3 → XP_006241370.1 protein phosphatase 1 regulatory subunit 12A isoform X1

UniProtKB/TrEMBL

A0A8I5Y8E5, A0A8I6ATJ5

Related

ENSRNOP00000078058.1, ENSRNOT00000108069.2

Conserved Domains (5) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:199 → 252

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:971 → 1069

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]
XM_006241313.3 → XP_006241375.1 protein phosphatase 1 regulatory subunit 12A isoform X5

UniProtKB/TrEMBL

D4ACS0

Conserved Domains (5) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:199 → 252

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:911 → 1009

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]
XM_039078253.2 → XP_038934181.1 protein phosphatase 1 regulatory subunit 12A isoform X2

UniProtKB/Swiss-Prot

Q10728, Q62937, Q9WU33

Conserved Domains (4) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:934 → 1032

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cd21944
Location:660 → 716

IPD_MYPT1; inhibitory phosphorylation domain of myosin phosphatase targeting subunit 1(MYPT1)
XM_039078254.2 → XP_038934182.1 protein phosphatase 1 regulatory subunit 12A isoform X7

UniProtKB/TrEMBL

D4ACS0

Conserved Domains (4) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:874 → 972

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cl40436
Location:609 → 655

IPD_PPP1R12; inhibitory phosphorylation domain of protein phosphatase 1 regulatory subunit 12 (PPP1R12) family
XM_006241312.3 → XP_006241374.1 protein phosphatase 1 regulatory subunit 12A isoform X4

UniProtKB/Swiss-Prot

Q10728, Q62937, Q9WU33

Conserved Domains (5) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:199 → 252

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:915 → 1013

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]
XM_039078255.2 → XP_038934183.1 protein phosphatase 1 regulatory subunit 12A isoform X9

UniProtKB/TrEMBL

D4ACS0

Conserved Domains (4) summary

PHA03095
Location:43 → 272

PHA03095; ankyrin-like protein; Provisional

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:855 → 953

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cd21944
Location:589 → 637

IPD_MYPT1; inhibitory phosphorylation domain of myosin phosphatase targeting subunit 1(MYPT1)
XM_006241316.5 → XP_006241378.1 protein phosphatase 1 regulatory subunit 12A isoform X12

UniProtKB/TrEMBL

A0A8I6ATJ5

Conserved Domains (4) summary

cd00204
Location:9 → 104

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam13637
Location:23 → 76

Ank_4; Ankyrin repeats (many copies)

pfam15898
Location:795 → 893

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:22 → 53

ANK; ANK repeat [structural motif]