OLE1 stearoyl-CoA 9-desaturase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: OLE1
Official Full Name: stearoyl-CoA 9-desaturase
Primary source: SGD:S000003023
Locus tag: YGL055W
See related: AllianceGenome:SGD:S000003023; FungiDB:YGL055W; VEuPathDB:YGL055W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MDM2
Summary: Enables electron transfer activity and stearoyl-CoA 9-desaturase activity. Involved in mitochondrion inheritance and unsaturated fatty acid biosynthetic process. Located in endoplasmic reticulum membrane. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 79. Orthologous to human SCD (stearoyl-CoA desaturase) and SCD5 (stearoyl-CoA desaturase 5). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See OLE1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (398628..400160)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Saccharomyces cerevisiae Delta9-desaturase Ole1 forms a supercomplex with Slc1 and Dga1.
Title: Saccharomyces cerevisiae Δ9-desaturase Ole1 forms a supercomplex with Slc1 and Dga1.
OLE1, which was identified as the only gene with a differential single-nucleotide polymorphism mutation site related to fatty acid metabolism, was significantly changed in response to ethanol. The role of OLE1 in membrane fluidity was positively validated in its overexpressed transformants. OLE1 lowered the rate of decline in membrane fluidity and thus enabled the yeast to better fight the deleterious effects of ethanol.
Title: Membrane Fluidity of Saccharomyces cerevisiae from Huangjiu (Chinese Rice Wine) Is Variably Regulated by OLE1 To Offset the Disruptive Effect of Ethanol.
A crucial role of OLE1 in thermotolerance was identified: the overexpression of OLE1 increased fatty acid unsaturation, and thereby helped counter lipid peroxidation caused by heat stress, rendering the yeast thermotolerant.
Title: CRISPR/Cas-based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance.
The OAF1 and OLE1 variants showed a non-additive genetic interaction, and affected cellular lipid metabolism. These results demonstrate that the molecular basis of trans-regulatory variation is diverse, highlighting the challenges in predicting which natural genetic variants affect gene expression
Title: DNA variants affecting the expression of numerous genes in trans have diverse mechanisms of action and evolutionary histories.
The results indicate that overexpression of OLE1 reduces oxidative stress induced by cadmium possibly through the inhibition of lipid peroxidation and protection of the cytoplasmic membrane from damage in yeast cells.
Title: OLE1 reduces cadmium-induced oxidative damage in Saccharomyces cerevisiae.
Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations.
Title: Regulation of yeast fatty acid desaturase in response to iron deficiency.
data suggest that the regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. The discovery of OLE1 involvement in membrane stability may indicate a novel defense strategy against cadmium stress [
Title: Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae.
Studied osmotic stress tolerance in Saccharomyces cerevisiae; found OLE1 overexpression improves stress tolerance and activates Hog1 pathway.
Title: Overexpression of OLE1 enhances stress tolerance and constitutively activates the MAPK HOG pathway in Saccharomyces cerevisiae.
role of OLE pathway in the maintenance of membrane fluidity
Title: Control of membrane fluidity: the OLE pathway in focus.
Ole1 partially relieves the growth defects of endoplasmic reticulum-mitochondria encounter structure -lacking cells.
Title: Identification of multi-copy suppressors for endoplasmic reticulum-mitochondria tethering proteins in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables electron transfer activity	IGI Inferred from Genetic Interaction more info	PubMed
enables electron transfer activity	ISA Inferred from Sequence Alignment more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables stearoyl-CoA 9-desaturase activity	IBA Inferred from Biological aspect of Ancestor more info
enables stearoyl-CoA 9-desaturase activity	IEA Inferred from Electronic Annotation more info
enables stearoyl-CoA 9-desaturase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in mitochondrion inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in unsaturated fatty acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in unsaturated fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in unsaturated fatty acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: stearoyl-CoA 9-desaturase

NP_011460.3

EC 1.14.19.1

Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

398628..400160

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180920.3 → NP_011460.3 TPA: stearoyl-CoA 9-desaturase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011460.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU86, E9P911, P21147

UniProtKB/TrEMBL

A6ZUC1, B3LHC4, C7GRJ1, N1P3L6

Conserved Domains (2) summary

COG1398
Location:92 → 385

OLE1; Fatty-acid desaturase [Lipid transport and metabolism]

COG5274
Location:408 → 510

CYB5; Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism]