RVB1 RuvB family ATP-dependent DNA helicase pontin [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RVB1
Official Full Name: RuvB family ATP-dependent DNA helicase pontin
Primary source: SGD:S000002598
Locus tag: YDR190C
See related: AllianceGenome:SGD:S000002598; FungiDB:YDR190C; VEuPathDB:YDR190C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TIH1; TIP49; TIP49A
Summary: Enables 5'-3' DNA helicase activity. Contributes to 3'-5' DNA helicase activity. Involved in box C/D snoRNP assembly; chromatin remodeling; and regulation of transcription by RNA polymerase II. Located in nucleus. Part of Ino80 complex; R2TP complex; and Swr1 complex. Orthologous to human RUVBL1 (RuvB like AAA ATPase 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RVB1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (840604..841995, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation.
Title: Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation.
results demonstrate the chaperone-like potential of Rvb1/Rvb2 and suggest a model where binding of multiple clients such as Ino80 stimulates ATP-driven cycling between hexamers and dodecamers, providing iterative opportunities for correct subunit assembly.
Title: Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones.
Data show that the 14 different polypeptide containing chromatin-remodeling complex SWR1 contains a single heterohexameric Rvb1/Rvb2 ring that, together with the catalytic subunit Swr1, brackets two independently assembled multisubunit modules.
Title: Molecular architecture of the ATP-dependent chromatin-remodeling complex SWR1.
purified endogenous untagged Rvb1/Rvb2 complexes were found as single hexameric rings, similar to the complexes assembled in vitro from purified untagged components
Title: Alternative oligomeric states of the yeast Rvb1/Rvb2 complex induced by histidine tags.
Pontin and reptin could acquire distinct structural states to regulate their broad functions as molecular motors and scaffolds for nucleic acids and proteins.
Title: Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes.
These results clearly establish the yeast Rvb1/2 complex as a heterohexameric ATP-dependent DNA helicase and highlight the possible roles played by the Rvb proteins within multiprotein complexes.
Title: Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex.
Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA; both proteins also interact with chromatin and play a role in recombination and repair by chromatin remodelling.
Title: Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-3' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in box C/D snoRNP assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in box C/D snoRNP assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Ino80 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ino80 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of NuA4 histone acetyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of R2TP complex	IBA Inferred from Biological aspect of Ancestor more info
part_of R2TP complex	IDA Inferred from Direct Assay more info	PubMed
part_of R2TP complex	IPI Inferred from Physical Interaction more info	PubMed
part_of Swr1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Swr1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Swr1 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of TTT Hsp90 cochaperone complex	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: RuvB family ATP-dependent DNA helicase pontin

NP_010476.1

EC 3.6.4.12

ATP-dependent DNA helicase, also known as pontin; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, the R2TP complex (Rvb1-Rvb2-Tah1-Pih1), and the TTT Hsp90 cochaperone complex that is involved in chromatin remodeling and telomeric chromatin regulation; involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly; member of the AAA+ and RuvB-like protein families, similar to Rvb2p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

840604..841995 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180498.1 → NP_010476.1 TPA: RuvB family ATP-dependent DNA helicase pontin [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010476.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSH3, Q03940

UniProtKB/TrEMBL

A6ZYE9, B3LG93, B5VG84, C7GN07, C8Z5A5, G2WAT9, N1P8D2

Conserved Domains (1) summary

COG1224
Location:4 → 463

TIP49; DNA helicase TIP49, TBP-interacting protein [Transcription]