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    ECM22 Ecm22p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850928, updated on 9-Dec-2024

    Summary

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    Official Symbol
    ECM22
    Official Full Name
    Ecm22p
    Primary source
    SGD:S000004218
    Locus tag
    YLR228C
    See related
    AllianceGenome:SGD:S000004218; FungiDB:YLR228C; VEuPathDB:YLR228C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in positive regulation of biosynthetic process; positive regulation of filamentous growth of a population of unicellular organisms in response to starvation; and positive regulation of sterol import. Located in membrane and perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See ECM22 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (600019..602463, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene tRNA-Leu Neighboring gene gag protein Neighboring gene gag-pol fusion protein Neighboring gene Rho family GTPase CDC42 Neighboring gene tRNA-Ile

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. UPC2A is required for high-level azole antifungal resistance in Candida glabrata. Whaley SG, et al. Antimicrob Agents Chemother, 2014 Aug. PMID 24867980, Free PMC Article
    2. Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency. Jordá T, et al. Environ Microbiol, 2022 Nov. PMID 36382795, Free PMC Article
    3. Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae. Marie C, et al. Fungal Genet Biol, 2008 Oct. PMID 18675371, Free PMC Article
    4. Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake. Shianna KV, et al. J Bacteriol, 2001 Feb. PMID 11208779, Free PMC Article
    5. Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2. Yang H, et al. Nat Commun, 2015 Feb 6. PMID 25655993

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cells lacking both ECM22 and UPC2 display strong mating defects whereas deletion of either gene has no effect. Ecm22 and Upc2 positively regulate basal expression of PRM1 and PRM4. These genes are strongly induced in response to mating pheromone, which is also largely dependent on ECM22 and UPC2.
      Title: Ecm22 and Upc2 regulate yeast mating through control of expression of the mating genes PRM1 and PRM4.
    2. Overexpression of either ECM22 or UPC2 leads to increased filamentation.
      Title: The zinc cluster proteins Upc2 and Ecm22 promote filamentation in Saccharomyces cerevisiae by sterol biosynthesis-dependent and -independent pathways.
    3. Perinuclear localization of Upc2p-GFP and Ecm22p-GFP was increased when ergosterol biosynthesis was blocked by azole drug treatment.
      Title: Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae.
    4. Upc2p and Ecm22p differ in their contributions to the regulation of their targets
      Title: Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription activator activity, RNA polymerase II-specific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription activator activity, RNA polymerase II-specific IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sequence-specific DNA binding HDA PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in positive regulation of ergosterol biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of sterol import IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Ecm22p
    NP_013329.1
    • Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      600019..602463 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182115.1 → NP_013329.1  TPA: Ecm22p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013329.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYN0, Q05958
      UniProtKB/TrEMBL
      B3RHB6, B5VNE5, C7GT26, C8ZDJ2, N1P1D4
      Conserved Domains (2) summary
      smart00066
      Location:39 → 80
      GAL4; GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain
      cl23766
      Location:508 → 638
      fungal_TF_MHR; fungal transcription factor regulatory middle homology region
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q05958.1

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