RNA14 cleavage polyadenylation factor subunit RNA14 [Saccharomyces cerevisiae S288C] - Gene

Summary

Official Symbol: RNA14
Official Full Name: cleavage polyadenylation factor subunit RNA14
Primary source: SGD:S000004665
Locus tag: YMR061W
See related: AllianceGenome:SGD:S000004665; FungiDB:YMR061W; VEuPathDB:YMR061W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to RNA binding activity. Involved in mRNA processing and response to DNA damage checkpoint signaling. Located in cytosol; mitochondrion; and nucleus. Part of mRNA cleavage stimulating factor complex. Orthologous to human CSTF3 (cleavage stimulation factor subunit 3). [provided by Alliance of Genome Resources, Dec 2024]
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Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

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CF IA is reconstituted from Rna14, Rna15, Pcf11, and Clp1 proteins in 2:2:1:1 stoichiometry.
Title: Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology.
Data show that mutation of residues within the minimal heterodimer region inhibit association of full-length Rna14p and Rna15p.
Title: Locked tether formation by cooperative folding of Rna14p monkeytail and Rna15p hinge domains in the yeast CF IA complex.
after inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p, and Gar1p delocalize from the nucleolus and accumulate in discrete nucleoplasmic foci
Title: Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus.
Study shows that overexpression of the Hrp1 or Rna14 subunits of the CF IA polyadenylation factor increases recognition of a weakened polyadenylation site.
Title: Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI.
Yeast cap binding factor impedes recruitment of CF I to weak termination sites.
Title: Yeast cap binding complex impedes recruitment of cleavage factor IA to weak termination sites.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
contributes_to RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in RNA 3'-end processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA 3'-end processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in response to DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of mRNA cleavage stimulating factor complex	IPI Inferred from Physical Interaction more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info

NP_013777.1

Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia

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