CDC53 cullin CDC53 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CDC53
Official Full Name: cullin CDC53
Primary source: SGD:S000002290
Locus tag: YDL132W
See related: AllianceGenome:SGD:S000002290; FungiDB:YDL132W; VEuPathDB:YDL132W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA replication origin binding activity and protein-macromolecule adaptor activity. Contributes to ubiquitin protein ligase activity. Involved in G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; and SCF-dependent proteasomal ubiquitin-dependent protein catabolic process. Part of SCF ubiquitin ligase complex. Orthologous to several human genes including CUL1 (cullin 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CDC53 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (224304..226751)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This ubiquitylation and degradation pathway acts on Met30 that is dissociated from Skp1. Unexpectedly, this pathway required the cullin component Cdc53/Cul1 but was independent of the other central SCF component Skp1
Title: Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.
A dual E3 mechanism for Rub1 ligation to Cdc53.
Title: A dual E3 mechanism for Rub1 ligation to Cdc53.
binding of substrate-specific adaptors triggers release of Cand1/Lag2, whereas subsequent neddylation of the cullin facilitates the removal and prevents re-association of Lag2/Cand1
Title: Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae.
The binding site for Dcn1 on Cdc53 is located approximately 18 A from the site of neddylation.
Title: Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation.
the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination
Title: Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein-macromolecule adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed
contributes_to ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
contributes_to ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G2/M transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in macromolecule catabolic process	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SCF ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
part_of cullin-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cullin CDC53

NP_010150.1

Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

224304..226751

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180191.1 → NP_010150.1 TPA: cullin CDC53 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010150.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRL7, Q12018

UniProtKB/TrEMBL

G2WC29, N1P5J1

Conserved Domains (1) summary

COG5647
Location:7 → 815

COG5647; Cullin, a subunit of E3 ubiquitin ligase [Posttranslational modification, protein turnover, chaperones]