RPN10 proteasome regulatory particle base subunit RPN10 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPN10
Official Full Name: proteasome regulatory particle base subunit RPN10
Primary source: SGD:S000001243
Locus tag: YHR200W
See related: AllianceGenome:SGD:S000001243; FungiDB:YHR200W; VEuPathDB:YHR200W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MCB1; SUN1
Summary: Enables K48-linked polyubiquitin modification-dependent protein binding activity. Involved in proteasome assembly and ubiquitin-dependent protein catabolic process. Part of proteasome regulatory particle, base subcomplex. Orthologous to human PSMD4 (proteasome 26S subunit ubiquitin receptor, non-ATPase 4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RPN10 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (499079..499885)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rpn10, but not ubiquitylated-Rpn10, binds Rpn9 in vitro: ubiquitylation functions to dissociate modified ubiquitin receptors from their targets, a function that promotes cyclic activity of ubiquitin receptors
Title: Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism.
Genetic, bioinformatics, biochemical and biophysical data show that attraction between this alpha1-conjugated ubiquitin and the HECT ubiquitin-binding patch pulls the alpha1-helix out of the interface, thereby promoting trimerization. Strikingly, trimerization renders the ubiquitin ligase inactive.
Title: Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.
functional yeast proteasomes have the capacity to associate and dissociate with Rpn10 and that Rpn10 monoubiquitination decreases the Rpn10-proteasome and Rpn10-Dsk2 associations.
Title: Rpn10 monoubiquitination orchestrates the association of the ubiquilin-type DSK2 receptor with the proteasome.
Mutations in this disordered region promote ubiquitin chain extension of Rpn10.
Title: An intrinsically disordered region of RPN10 plays a key role in restricting ubiquitin chain elongation in RPN10 monoubiquitination.
X-ray analysis revealed that these crystals of Rpn10 belonged to the monoclinic system C2, with unit-cell parameters a = 107.3, b = 49.7, c = 81.3 A, alpha = gamma = 90.0, beta = 130.5 degrees
Title: Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10.
On the basis of the mutual positions of Rpn10 and Rpn13, we propose a model for polyubiquitin binding to the 26S proteasome
Title: Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy.
It is conceivable that Rpn10/Rpn11 interaction couples proteasome assembly to substrate binding.
Title: Synthetic lethality of rpn11-1 rpn10Δ is linked to altered proteasome assembly and activity.
Data report that Rpn10 is monoubiquitinated in vivo and that this modification has profound effects on proteasome function.
Title: Monoubiquitination of RPN10 regulates substrate recruitment to the proteasome.
Data describe formation of ternary complexes of Dsk2 and Rpn10 with (poly)ubiquitin and found that, depending on the ubiquitin chain length, the orientation of the resulting complex is entirely different, providing for alternate signals.
Title: Together, Rpn10 and Dsk2 can serve as a polyubiquitin chain-length sensor.
The yeast homologue of S5a, Rpn10, enhanced the proteasomal degradation of troponin I to the same extent as S5a.
Title: S5a promotes protein degradation by blocking synthesis of nondegradable forked ubiquitin chains.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables K48-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in proteasome assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of proteasome regulatory particle, base subcomplex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome regulatory particle, base subcomplex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle base subunit RPN10

NP_012070.3

Proteasome polyubiquitin receptor; non-ATPase subunit of the 19S regulatory particle (RP) of the 26S proteasome that links the RP base to the lid; N-terminus plays a role in the assembly of the regulatory particle (RP); ubiquitin-interacting motif selectively binds to polyubiquitin chains; homolog of the mammalian S5a protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

499079..499885

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179331.3 → NP_012070.3 TPA: proteasome regulatory particle base subunit RPN10 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012070.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLE9, P38886

UniProtKB/TrEMBL

A6ZTA7, B3LSW1, B5VKC9, C7GRM2, C8ZA01, N1P1C6

Conserved Domains (1) summary

COG5148
Location:1 → 236

RPN10; 26S proteasome regulatory complex, subunit RPN10/PSMD4 [Posttranslational modification, protein turnover, chaperones]