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    PRP5 DEAD-box RNA helicase PRP5 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852539, updated on 9-Dec-2024

    Summary

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    Official Symbol
    PRP5
    Official Full Name
    DEAD-box RNA helicase PRP5
    Primary source
    SGD:S000000441
    Locus tag
    YBR237W
    See related
    AllianceGenome:SGD:S000000441; FungiDB:YBR237W; VEuPathDB:YBR237W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    RNA5
    Summary
    Enables ATP-dependent activity, acting on RNA. Involved in mRNA branch site recognition. Located in nucleus. Orthologous to human DDX46 (DEAD-box helicase 46). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See PRP5 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_001134.8 (691969..694518)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Vhc1p Neighboring gene mRNA (guanine-N7)-methyltransferase Neighboring gene uncharacterized protein Neighboring gene Ert1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. PRP5: a helicase-like protein required for mRNA splicing in yeast. Dalbadie-McFarland G, et al. Proc Natl Acad Sci U S A, 1990 Jun. PMID 2349233, Free PMC Article
    2. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA. O'Day CL, et al. J Biol Chem, 1996 Dec 27. PMID 8969184
    3. Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly. Zhang ZM, et al. Cell Rep, 2013 Dec 12. PMID 24290758
    4. Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. Abu Dayyeh BK, et al. J Biol Chem, 2002 Jun 7. PMID 11927574
    5. Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly. Beier DH, et al. Nucleic Acids Res, 2019 Nov 18. PMID 31712821, Free PMC Article

    See all (63) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural insights into how Prp5 proofreads the pre-mRNA branch site.
      Title: Structural insights into how Prp5 proofreads the pre-mRNA branch site.
    2. Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
      Title: Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
    3. different HSH155 alleles elicit disparate effects on splicing: some increase the fidelity of BS selection while others decrease fidelity. Our data support a model wherein conformational changes in SF3b1 promote U2 association with the BS independently of the action of the DEAD-box ATPase Prp5. We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing
      Title: SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.
    4. Taken together, our results indicate that the Psis in U2 snRNA contribute to pre-mRNA splicing by directly altering the binding/ATPase activity of Prp5.
      Title: Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly.
    5. Prp5 binds to the spliceosome in association with U2 by interacting with the BSL and is released upon the base-pairing of U2 with the branch site to allow the recruitment of the tri-snRNP.
      Title: A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on RNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA branch site recognition IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ribonucleoprotein complex biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DEAD-box RNA helicase PRP5
    NP_009796.1
    • RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      691969..694518
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178585.1 → NP_009796.1  TPA: DEAD-box RNA helicase PRP5 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009796.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQN3, P21372
      UniProtKB/TrEMBL
      G2W9P0
      Conserved Domains (3) summary
      COG0513
      Location:227 → 731
      SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
      cd17953
      Location:244 → 468
      DEADc_DDX46; DEAD-box helicase domain of DEAD box protein 46
      cd22474
      Location:749 → 837
      KH-I_PRP5_like; type I K homology (KH) RNA-binding domain found in fungal pre-mRNA-processing ATP-dependent RNA helicase PRP5 and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P21372.1

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