hlyE hemolysin E [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: hlyE
Official Full Name: hemolysin E
Primary source: ECOCYC:G6619
Locus tag: b1182
See related: ASAP:ABE-0003962
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: clyA; ECK1170; hpr; sheA; ycgD
Summary: HylE is secreted but has no signal peptide. [More information is available at EcoGene: EG13243]. Hemolysin E causes lysis of mammalian cells . [More information is available at EcoCyc: G6619].
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Genomic context

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Sequence:: NC_000913.3 (1229483..1230394, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury.
Title: Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury.
Membrane-triggered conformational transition of ClyA has been caprured and reported.
Title: Capturing the Membrane-Triggered Conformational Transition of an α-Helical Pore-Forming Toxin.
The assembly dynamics of the cytolytic pore toxin ClyA has been reported.
Title: The assembly dynamics of the cytolytic pore toxin ClyA.
These findings show that the clyA+ locus is expressed at an elevated level in the uropathogenic Escherichia coli strain and the authors conclude that this is at least in part due to the effect of the SfaX/PapX transcriptional regulators.
Title: Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains.
ClyA is delivered to the target host cell in an oligomeric conformation within outer membrane vesicles (OMVs), our findings suggest ClyA forms a prepore oligomeric structure independently of the lipid membrane within the OMV.
Title: A non-classical assembly pathway of Escherichia coli pore-forming toxin cytolysin A.
Novel site(s) in HlyB responsible for enhancing secretion of cyclodextrin glucanotransferase in E. coli.
Title: Enhanced secretory production of hemolysin-mediated cyclodextrin glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system.
results failed to show a positive correlation with SIDS, instead proving that clyA and irp2 genes were common to the infant intestinal E. coli
Title: The frequency of molecular detection of virulence genes encoding cytolysin A, high-pathogenicity island and cytolethal distending toxin of Escherichia coli in cases of sudden infant death syndrome does not differ from that in other infant deaths and healthy infants.
Data Show that hlyE expression in Escherichia coli was controlled by alternate SlyA and H-NS nucleoprotein complexes.
Title: Alternate SlyA and H-NS nucleoprotein complexes control hlyE expression in Escherichia coli K-12.
Circular dichroism and fluorescence energy transfer analyses show that HlyE protomers retain an alpha-helical structure when oligomerized to form a pore consisting of parallel HlyE protomers.
Title: The formation and structure of Escherichia coli K-12 haemolysin E pores.
N-terminus verified by Edman degradation on mature peptide
Title: Analysis of the SlyA-controlled expression, subcellular localization and pore-forming activity of a 34 kDa haemolysin (ClyA) from Escherichia coli K-12.

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General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables toxin activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in hemolysis in another organism	IEA Inferred from Electronic Annotation more info
involved_in killing of cells of another organism	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within modulation of apoptotic process in another organism	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in host cell plasma membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in periplasmic space	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: hemolysin E

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1229483..1230394 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415700.4 hemolysin E [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415700.4

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A1X3JJK2

Conserved Domains (1) summary

PRK11376
Location:1 → 303

hlyE; hemolysin HlyE