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    trmL tRNA (cytidine/uridine-2'-O)-ribose methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948119, updated on 3-Dec-2024

    Summary

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    Official Symbol
    trmL
    Official Full Name
    tRNA (cytidine/uridine-2'-O)-ribose methyltransferase
    Primary source
    ECOCYC:EG11888
    Locus tag
    b3606
    See related
    ASAP:ABE-0011790
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3596; yibK
    Summary
    otherPDB (H. influenzae): [More information is available at EcoGene: EG11888]. YibK belongs to the SPOUT superfamily of methyltransferases . [More information is available at EcoCyc: EG11888].
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    Genomic context

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    Sequence:
    NC_000913.3 (3781215..3781688)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene lactate/glycolate:H(+) symporter LldP Neighboring gene DNA-binding transcriptional dual regulator LldR Neighboring gene L-lactate dehydrogenase Neighboring gene serine acetyltransferase Neighboring gene glycerol-3-phosphate dehydrogenase Neighboring gene protein export chaperone SecB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The tRNA recognition mechanism of the minimalist SPOUT methyltransferase, TrmL. Liu RJ, et al. Nucleic Acids Res, 2013 Sep. PMID 23804755, Free PMC Article
    2. Mutants of Escherichia coli K-12 with an altered glutamyl-transfer ribonucleic acid synthetase. Murgola EJ, et al. J Bacteriol, 1970 Jul. PMID 4912521, Free PMC Article
    3. Streptomycin-suppressible lethal mutations in Escherichia coli. Murgola EJ, et al. J Bacteriol, 1970 Jul. PMID 4912524, Free PMC Article
    4. Folding studies on a knotted protein. Mallam AL, et al. J Mol Biol, 2005 Mar 11. PMID 15713490
    5. Tying the knot that binds. Gloss LM. Structure, 2007 Jan. PMID 17223526

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that only pyrimidines at the wobble position are recognized by C/U34 2'-O-methyltransferase (EcTrmL).
      Title: Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2'-O-methyltransferase.
    2. The results suggest that TrmL functions as a homodimer by using the conserved C-terminal half of the SPOUT domain for catalysis, whereas residues from the less-conserved N-terminal half of the other subunit participate in tRNA recognition.
      Title: The tRNA recognition mechanism of the minimalist SPOUT methyltransferase, TrmL.
    3. Loss of YibK methylation reduces the efficiency of codon-wobble base interaction, as demonstrated in an amber suppressor supP system.
      Title: YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables S-adenosylmethionine-dependent methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA (cytidine(34)-2'-O)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in wobble position cytosine ribose methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within wobble position cytosine ribose methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in wobble position uridine ribose methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within wobble position uridine ribose methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    tRNA (cytidine/uridine-2'-O)-ribose methyltransferase
    NP_418063.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3781215..3781688
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418063.1 tRNA (cytidine/uridine-2'-O)-ribose methyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418063.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AMS0
      Conserved Domains (1) summary
      PRK10358
      Location:1 → 157
      PRK10358; tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL
    Nucleotide Protein
    Heading Accession and Version

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