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    THI4 thiamine thiazole synthase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853047, updated on 27-Dec-2024

    Summary

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    Official Symbol
    THI4
    Official Full Name
    thiamine thiazole synthase
    Primary source
    SGD:S000003376
    Locus tag
    YGR144W
    See related
    AllianceGenome:SGD:S000003376; FungiDB:YGR144W; VEuPathDB:YGR144W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    ESP35; MOL1
    Summary
    Enables cysteine-dependent adenosine diphosphate thiazole synthase activity and ferrous iron binding activity. Involved in mitochondrial genome maintenance; thiamine biosynthetic process; and thiazole biosynthetic process. Located in cytosol. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See THI4 in Genome Data Viewer
    Location:
    chromosome: VII
    Exon count:
    1
    Sequence:
    Chromosome: VII; NC_001139.9 (780399..781379)

    Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene beta-glucan synthesis-associated protein SKN1 Neighboring gene tRNA-Gly Neighboring gene ribosome biosynthesis protein ENP2 Neighboring gene Ecl1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001139.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Fusarium oxysporum sti35 gene functions in thiamine biosynthesis and oxidative stress response. Ruiz-Roldán C, et al. Fungal Genet Biol, 2008 Jan. PMID 17964830
    2. MOL1, a Saccharomyces cerevisiae gene that is highly expressed in early stationary phase during growth on molasses. Praekelt UM, et al. Yeast, 1992 Sep. PMID 1441749
    3. Regulation of THI4 (MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae. Praekelt UM, et al. Yeast, 1994 Apr. PMID 7941734
    4. Thiamin biosynthesis in eukaryotes: characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis. Chatterjee A, et al. J Am Chem Soc, 2006 Jun 7. PMID 16734458, Free PMC Article
    5. Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae. Jurgenson CT, et al. Biochemistry, 2006 Sep 19. PMID 16964967

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. observations confirm NAD as the substrate for THI4 and elucidate the early steps of this unique biosynthesis of the thiazole moiety of thiamin in eukaryotes
      Title: Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.
    2. CyPBP37 is characterized as an abundant cytosolic protein homologous with yeast Thi4, and with a functional NAD-binding site.
      Title: Neurospora crassa CyPBP37: a cytosolic stress protein that is able to replace yeast Thi4p function in the synthesis of vitamin B1.
    3. structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 A resolution
      Title: Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables cysteine-dependent adenosine diphosphate thiazole synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine-dependent adenosine diphosphate thiazole synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ferrous iron binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pentosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in mitochondrial genome maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in thiamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in thiazole biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in thiazole biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in thiazole biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    thiamine thiazole synthase
    NP_011660.1
    • Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001139.9 Reference assembly

      Range
      780399..781379
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181273.1 → NP_011660.1  TPA: thiamine thiazole synthase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_011660.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VUS4, P32318
      UniProtKB/TrEMBL
      A6ZUF6, B3LI86, B5VJ88, C7GSX8, G2WEM4, N1P438
      Conserved Domains (1) summary
      TIGR00292
      Location:45 → 325
      TIGR00292; thiazole biosynthesis enzyme
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32318.1

    Gene LinkOut

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