Ppp1r9b protein phosphatase 1, regulatory subunit 9B [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ppp1r9bprovided by RGD
Official Full Name: protein phosphatase 1, regulatory subunit 9Bprovided by RGD
Primary source: RGD:632281
See related: EnsemblRapid:ENSRNOG00000052113 AllianceGenome:RGD:632281
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Neb2
Summary: Enables several functions, including D2 dopamine receptor binding activity; enzyme binding activity; and transmembrane transporter binding activity. Involved in several processes, including cellular response to epidermal growth factor stimulus; pallium development; and positive regulation of protein localization to cell periphery. Located in several cellular components, including dendritic spine; growth cone; and neuronal cell body. Is active in glutamatergic synapse; postsynaptic density; and spine apparatus. Is extrinsic component of postsynaptic membrane. Biomarker of Parkinson's disease. Orthologous to human PPP1R9B (protein phosphatase 1 regulatory subunit 9B). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 458.6), Lung (RPKM 210.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q26

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (80434888..80450919)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (79938055..79954085)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (82800704..82816735)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PP1 bound on spinophilin, a close homolog of neurabin and another major synaptic PP1 anchoring protein, does not play a role in long term depression [LTD] induction, which suggests that neurabin plays a privileged role in nanodomain targeting of PP1 in LTD induction.
Title: Distinct Roles of Protein Phosphatase 1 Bound on Neurabin and Spinophilin and Its Regulation in AMPA Receptor Trafficking and LTD Induction.
At 1 d after acute foodshake-stress Spinophilin was significantly increased in At 1 d after desipramine-treated animals as compared to vehicle-treated animals.
Title: The expression of plasticity-related genes in an acute model of stress is modulated by chronic desipramine in a time-dependent manner within medial prefrontal cortex.
The guanine nucleotide exchange factor (GEF) Asef2 promotes dendritic spine formation via Rac activation and spinophilin-dependent targeting.
Title: The guanine nucleotide exchange factor (GEF) Asef2 promotes dendritic spine formation via Rac activation and spinophilin-dependent targeting.
Aging-induced proteostatic changes in the rat hippocampus identify ARP3, NEB2 and BRAG2 as a molecular circuitry for cognitive impairment.
Title: Aging-induced proteostatic changes in the rat hippocampus identify ARP3, NEB2 and BRAG2 as a molecular circuitry for cognitive impairment.
Data show that the PP1:spinophilin holoenzyme is dynamic in solution, which allows for an increased capture radius of spinophilin and is likely important for its biological role.
Title: Flexibility in the PP1:spinophilin holoenzyme.
The current results suggest a possible role for spinophilin in neuronal plasticity induced by environmental enrichment.
Title: Enriched environment increases spinophilin mRNA expression and spinophilin immunoreactive dendritic spines in hippocampus and cortex.
expression of spinophilin (SPL) could enhance the regulatory function of RGS8 on the M3-mAChR system, but the acceleration function of RGS8 on the M2-mediated signaling could not be enhanced by SPL
Title: Effects of spinophilin on the function of RGS8 regulating signals from M2 and M3-mAChRs.
selective targeting of PP1gamma1 to F-actin by neurabins in intact cells requires both the canonical PP1-binding motif and an auxiliary PP1gamma1-selectivity determinant
Title: Selective targeting of the gamma1 isoform of protein phosphatase 1 to F-actin in intact cells requires multiple domains in spinophilin and neurabin.
Associatively conditioned rats had more spinophilin in their dendritic spines than controls; changes in the number of dendritic spines must occur during learning and account in part for memory.
Title: Associative Pavlovian conditioning leads to an increase in spinophilin-immunoreactive dendritic spines in the lateral amygdala.
Regions of spinophilin that lack the revised Actin-binding Domain (ABD) do not co-localize with phalloidin-labeled actin, suggesting that the ABD contributes to directing the subcellular distribution of spinophilin.
Title: The identification of a second actin-binding region in spinophilin/neurabin II.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BE117171 (e-PCR)
- UniSTS:248651

BF405560 (e-PCR)
- UniSTS:247937

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables D2 dopamine receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables nucleic acid binding	EXP Inferred from Experiment more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase 1 binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase 1 binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane transporter binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament depolymerization	IEP Inferred from Expression Pattern more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in calcium-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to epidermal growth factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to estradiol stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to morphine	ISO Inferred from Sequence Orthology more info
involved_in cellular response to morphine	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to peptide	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cerebral cortex development	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within dendrite development	ISO Inferred from Sequence Orthology more info
involved_in developmental process involved in reproduction	IEP Inferred from Expression Pattern more info	PubMed
involved_in filopodium assembly	ISO Inferred from Sequence Orthology more info
involved_in filopodium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in hippocampus development	IEP Inferred from Expression Pattern more info	PubMed
involved_in learning	IEP Inferred from Expression Pattern more info	PubMed
involved_in male mating behavior	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in neuron projection development	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of protein localization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein localization to actin cortical patch	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to actin cytoskeleton	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to cell periphery	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of opioid receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of opioid receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of postsynapse assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in reproductive system development	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to L-phenylalanine derivative	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to amino acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to amphetamine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to estradiol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to immobilization stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to kainic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nicotine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to prostaglandin E	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to steroid hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in adherens junction	IEA Inferred from Electronic Annotation more info
located_in cortical actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasmic side of dendritic spine plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine head	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine membrane	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine neck	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	ISO Inferred from Sequence Orthology more info
located_in filopodium	ISS Inferred from Sequence or Structural Similarity more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in ruffle membrane	ISO Inferred from Sequence Orthology more info
located_in ruffle membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in spine apparatus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neurabin-2

Names: PP1bp134; neurabin-II; neural tissue-specific F-actin-binding protein II; nuerabin 2; p130; spinophilin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053474.2 → NP_445926.1 neurabin-2

See identical proteins and their annotated locations for NP_445926.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

O35274

UniProtKB/TrEMBL

B1H262

Related

ENSRNOP00000074026.1, ENSRNOT00000089497.3

Conserved Domains (3) summary

smart00228
Location:493 → 582

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

pfam07106
Location:682 → 763

TBPIP; Tat binding protein 1(TBP-1)-interacting protein (TBPIP)

cl23943
Location:745 → 811

PCRF; PCRF domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

80434888..80450919

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039086990.2 → XP_038942918.1 neurabin-2 isoform X1

UniProtKB/TrEMBL

B1H262

Conserved Domains (2) summary

cd00992
Location:497 → 580

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

pfam17817
Location:426 → 489

PDZ_5; PDZ domain