YEN1 crossover junction endodeoxyribonuclease [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YEN1
Official Full Name: crossover junction endodeoxyribonuclease
Primary source: SGD:S000000843
Locus tag: YER041W
See related: AllianceGenome:SGD:S000000843; FungiDB:YER041W; VEuPathDB:YER041W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables crossover junction DNA endonuclease activity and double-stranded DNA 5'-3' DNA exonuclease activity. Involved in DNA repair. Located in cytoplasm. Is active in nucleus. Orthologous to human GEN1 (GEN1 Holliday junction 5' flap endonuclease). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See YEN1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (232461..234740)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors.
Title: Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors.
SUMO-mediated recruitment allows timely function of the Yen1 nuclease in mitotic cells.
Title: SUMO-mediated recruitment allows timely function of the Yen1 nuclease in mitotic cells.
Canonical and novel non-canonical activities of the Holliday junction resolvase Yen1.
Title: Canonical and novel non-canonical activities of the Holliday junction resolvase Yen1.
The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis.
Title: The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis.
Yen1 and Uls1 displayed a physical interaction. This interaction was strengthened by SUMO-modification of Yen1.
Title: Helicase/SUMO-targeted ubiquitin ligase Uls1 interacts with the Holliday junction resolvase Yen1.
Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1.
Title: Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1.
Active suppression of Yen1 function, and by inference also of Mus81-Mms4(EME1) and Slx1-Slx4(BTBD12) resolvases, avoids precocious resolution of recombination intermediates to enable meiotic crossover patterning.
Title: Regulated Crossing-Over Requires Inactivation of Yen1/GEN1 Resolvase during Meiotic Prophase I.
Unresolved recombination intermediates are a source of mitotic sister chromatid bridges, and Mus81-Mms4 and Yen1 play a central role in their resolution in vivo.
Title: Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions.
Master cell-cycle regulators, cyclin-dependent kinase (Cdk) and Cdc14 phosphatase, control the actions of Yen1 during mitosis.
Title: Dual control of Yen1 nuclease activity and cellular localization by Cdk and Cdc14 prevents genome instability.
Have refined the substrate specificity of budding yeast Cdc14 and, using this insight, identified the Holliday junction resolvase Yen1 as a DNA repair target of Cdc14.
Title: The Cdk/cDc14 module controls activation of the Yen1 holliday junction resolvase to promote genome stability.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-3' exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables crossover junction DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables crossover junction DNA endonuclease activity	IEA Inferred from Electronic Annotation more info
enables crossover junction DNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables double-stranded DNA 5'-3' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables endonuclease activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA repair	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: crossover junction endodeoxyribonuclease

NP_010959.2

Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

232461..234740

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178932.2 → NP_010959.2 TPA: crossover junction endodeoxyribonuclease [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010959.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLU1, P40028, Q02463

UniProtKB/TrEMBL

C7GSI8, C8Z744

Conserved Domains (2) summary

cd09906
Location:286 → 388

H3TH_YEN1; H3TH domain of Yeast Endonuclease 1, a structure-specific, divalent-metal-ion dependent, 5' nuclease

cd09870
Location:2 → 275

PIN_YEN1; FEN-like PIN domains of Saccharomyces cerevisiae endonuclease 1 (YEN1), Chaetomium thermophilum junction-resolving enzyme GEN1, and fungal homologs