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    SPT14 phosphatidylinositol N-acetylglucosaminyltransferase SPT14 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855928, updated on 9-Dec-2024

    Summary

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    Official Symbol
    SPT14
    Official Full Name
    phosphatidylinositol N-acetylglucosaminyltransferase SPT14
    Primary source
    SGD:S000006096
    Locus tag
    YPL175W
    See related
    AllianceGenome:SGD:S000006096; FungiDB:YPL175W; VEuPathDB:YPL175W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    CWH6; GPI3
    Summary
    Enables phosphatidylinositol N-acetylglucosaminyltransferase activity. Predicted to be involved in GPI anchor biosynthetic process. Located in endoplasmic reticulum. Used to study paroxysmal nocturnal hemoglobinuria. Human ortholog(s) of this gene implicated in multiple congenital anomalies-hypotonia-seizures syndrome 2 and paroxysmal nocturnal hemoglobinuria. Orthologous to human PIGA (phosphatidylinositol glycan anchor biosynthesis class A). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See SPT14 in Genome Data Viewer
    Location:
    chromosome: XVI
    Exon count:
    2
    Sequence:
    Chromosome: XVI; NC_001148.4 (218630..220088)

    Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene Cup9p Neighboring gene Tre1p Neighboring gene Nip100p Neighboring gene mitochondrial 54S ribosomal protein YmL40

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001148.4

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Restrictive glycosylphosphatidylinositol anchor synthesis in cwh6/gpi3 yeast cells causes aberrant biogenesis of cell wall proteins. Vossen JH, et al. J Bacteriol, 1997 Apr. PMID 9079905, Free PMC Article
    2. Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis. Kostova Z, et al. Biochem J, 2000 Sep 15. PMID 10970797, Free PMC Article
    3. Identification of SPT14/CWH6 as the yeast homologue of hPIG-A, a gene involved in the biosynthesis of GPI anchors. Vossen JH, et al. Biochim Biophys Acta, 1995 Apr 13. PMID 7727533
    4. The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis. Schönbächler M, et al. EMBO J, 1995 Apr 18. PMID 7737116, Free PMC Article
    5. Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly. Kostova Z, et al. Eur J Biochem, 2003 Nov. PMID 14622279

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatidylinositol N-acetylglucosaminyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables phosphatidylinositol N-acetylglucosaminyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatidylinositol N-acetylglucosaminyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in GPI anchor biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in GPI anchor biosynthetic process IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in GPI anchor biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol HDA PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    phosphatidylinositol N-acetylglucosaminyltransferase SPT14
    NP_015150.2
    • UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001148.4 Reference assembly

      Range
      218630..220088
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183989.1 → NP_015150.2  TPA: phosphatidylinositol N-acetylglucosaminyltransferase SPT14 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_015150.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B3LKQ3, B5VSZ6, D6W3J3, P32363, Q08918
      UniProtKB/TrEMBL
      C8ZIK3, G2WP07, N1P2P7
      Conserved Domains (1) summary
      cd03796
      Location:4 → 408
      GT4_PIG-A-like; phosphatidylinositol N-acetylglucosaminyltransferase subunit A and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32363.4

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