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    DNAlig4 DNA ligase 4 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 32322, updated on 9-Dec-2024

    Summary

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    Official Symbol
    DNAlig4provided by FlyBase
    Official Full Name
    DNA ligase 4provided by FlyBase
    Primary source
    FLYBASE:FBgn0030506
    Locus tag
    Dmel_CG12176
    See related
    AllianceGenome:FB:FBgn0030506
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG12176; Dmel\CG12176; lig4; Lig4; LIG4; ligase IV; ligaseIV; ligIV; RE37186
    Summary
    Predicted to enable ATP binding activity; DNA binding activity; and DNA ligase (ATP) activity. Involved in DNA metabolic process and response to ionizing radiation. Predicted to be part of DNA ligase IV complex and DNA-dependent protein kinase-DNA ligase 4 complex. Is expressed in organism and spermatozoon. Human ortholog(s) of this gene implicated in several diseases, including DNA ligase IV deficiency; colorectal cancer; multiple myeloma; pancreatic cancer; and prostate cancer. Orthologous to human LIG4 (DNA ligase 4). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
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    Genomic context

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    See DNAlig4 in Genome Data Viewer
    Location:
    12B2-12B2; 1-45 cM
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (13664584..13667811, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (13558617..13561844, complement)

    Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene NFAT nuclear factor Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster. Joshi R, et al. J Appl Genet, 2022 Feb. PMID 34817771, Free PMC Article
    2. Secondary structure forming sequences drive SD-MMEJ repair of DNA double-strand breaks. Khodaverdian VY, et al. Nucleic Acids Res, 2017 Dec 15. PMID 29121353, Free PMC Article
    3. Recovery of Alternative End-Joining Repair Products From Drosophila Embryos. Hanscom T, et al. Methods Enzymol, 2018. PMID 29523244
    4. Characterization of sequence contexts that favor alternative end joining at Cas9-induced double-strand breaks. Hanscom T, et al. Nucleic Acids Res, 2022 Jul 22. PMID 35819195, Free PMC Article
    5. Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. Beumer KJ, et al. G3 (Bethesda), 2013 Apr 9. PMID 23550125, Free PMC Article

    See all (80) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that when both spnA and lig4 were mutated and a circular donor was provided, the frequency of ry mutations was still high and no HR products were recovered.
      Title: Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila.
    2. Lig4 is required for repair of DNA double-strand breaks induced by P-element excision.
      Title: Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in Drosophila.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables ATP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA ligase (ATP) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA ligase (ATP) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA ligase (ATP) activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA ligation involved in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair via nonhomologous end joining IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in double-strand break repair via nonhomologous end joining IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in nucleotide-excision repair, DNA gap filling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in replication fork processing IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in response to ionizing radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere maintenance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA ligase IV complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of DNA-dependent protein kinase-DNA ligase 4 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    DNA ligase 4
    Names
    CG12176-PA
    DNA ligase IV
    DNAlig4-PA
    ligase 4
    ligase IV
    ligase4
    NP_572907.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_004354.4 Reference assembly

      Range
      13664584..13667811 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_132679.3 → NP_572907.1  DNA ligase 4 [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_572907.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q8MS06, Q9VYA5
      Related
      FBpp0073678
      Conserved Domains (5) summary
      cd07968
      Location:469 → 607
      OBF_DNA_ligase_IV; The Oligonucleotide/oligosaccharide binding (OB)-fold domain of ATP-dependent DNA ligase IV is a DNA-binding module that is part of the catalytic core unit
      cd07903
      Location:249 → 466
      Adenylation_DNA_ligase_IV; Adenylation domain of DNA Ligase IV
      smart00292
      Location:668 → 745
      BRCT; breast cancer carboxy-terminal domain
      TIGR00574
      Location:84 → 613
      dnl1; DNA ligase I, ATP-dependent (dnl1)
      pfam04675
      Location:10 → 197
      DNA_ligase_A_N; DNA ligase N terminus
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8MS06 Q8MS06
    Q9VYA5 Q9VYA5

    Gene LinkOut

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