tum tumbleweed [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: tumprovided by FlyBase
Official Full Name: tumbleweedprovided by FlyBase
Primary source: FLYBASE:FBgn0086356
Locus tag: Dmel_CG13345
See related: AllianceGenome:FB:FBgn0086356
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 13345; acGAP; AcGAP; CG13345; Dmel\CG13345; DRacGAP; eon; i249; RacGap; RacGAP; racGAP50; racGAP50c; racGAP50C; RacGap50C; RacGAP50c; RacGAP50C; RhoGAP-50C14; Tum; TUM; Tumbleweed
Summary: Predicted to enable GTPase activator activity. Involved in several processes, including Rho protein signal transduction; larval somatic muscle development; and negative regulation of signal transduction. Located in cleavage furrow; nucleus; and perinuclear region of cytoplasm. Part of centralspindlin complex. Colocalizes with cortical microtubule; midbody; and spindle. Is expressed in embryonic/larval hypodermal muscle cell and organism. Human ortholog(s) of this gene implicated in congenital dyserythropoietic anemia. Orthologous to human RACGAP1 (Rac GTPase activating protein 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 50C6-50C6; 2-68 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (13856150..13858689, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (9743655..9746194, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Centralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding.
Title: Centralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding.
RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.
Data show that GTPase-activating protein (Tum/RacGAP) is required for the motor activity of kinesin-6 motor (Pav/kinesin-6).
Title: Tum/RacGAP functions as a switch activating the Pav/kinesin-6 motor.
Study demonstrates the emerging role of centralspindlin (complex of two proteins Tum and Pav) in regulating cell migration and cell adhesion in addition to its previously known function during cytokinesis.
Title: Centralspindlin is required for thorax development during Drosophila metamorphosis.
Results identify two modulators of the Wnt pathway, Tum and Pav, that have shared functions in cell division, which hints at a possible link between cytokinesis and Wnt activity during tumorigenesis.
Title: Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.
a new domain of Polo kinase, termed the intermediate domain, interacts directly with RacGAP50C and that Polo kinase is essential for localization of the KLP-RacGAP centralspindlin complex to the cell equator and spindle midzone.
Title: Polo kinase interacts with RacGAP50C and is required to localize the cytokinesis initiation complex.
mislocalization of RacGAP50C in pavarotti mutants is sufficient to redistribute gamma-tubulin to the muscle fiber ends
Title: RacGAP50C directs perinuclear gamma-tubulin localization to organize the uniform microtubule array required for Drosophila myotube extension.
in addition to its role in activating RhoA signalling, RacGAP50C also controls the proper assembly of the actomyosin ring by interacting with Anillin at the cleavage furrow
Title: Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site.
the RacGap50C gene product participates in the negative regulation of Wg pathway activity
Title: RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development.
A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis.
Title: A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Rho protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in larval somatic muscle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of protein location in cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle midzone assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of epidermal growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of stem cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in somatic muscle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in wound healing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of centralspindlin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of centralspindlin complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cleavage furrow	IBA Inferred from Biological aspect of Ancestor more info
located_in cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cortical microtubule	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with midbody	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with spindle	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle midzone	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: tumbleweed

Names: CG13345-PA; RacGap50C; enhancer of naked; tum-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

13856150..13858689 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_137068.4 → NP_610912.2 tumbleweed [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_610912.2

Status: REVIEWED

UniProtKB/TrEMBL

A1Z9I5, Q9N9Z9

Related

FBpp0086767

Conserved Domains (2) summary

cd00029
Location:319 → 367

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd04382
Location:381 → 570

RhoGAP_MgcRacGAP; RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin ...