Hrs Hepatocyte growth factor regulated tyrosine kinase substrate [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Hrsprovided by FlyBase
Official Full Name: Hepatocyte growth factor regulated tyrosine kinase substrateprovided by FlyBase
Primary source: FLYBASE:FBgn0031450
Locus tag: Dmel_CG2903
See related: AllianceGenome:FB:FBgn0031450
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG2903; dHrs; Dmel\CG2903; DmHRS; hrs; HRS; HRS-2; l(2)23AB5; l(2)23Ad; l(2)23Ad(Hrs); vps27; Vps27
Summary: Enables ubiquitin binding activity. Involved in several processes, including endosomal transport; regulation of epithelial cell migration; and regulation of signal transduction. Located in endosome. Is expressed in adult head; embryonic/larval hemocyte; and organism. Orthologous to human HGS (hepatocyte growth factor-regulated tyrosine kinase substrate). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 23A3-23A3; 2-7 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (2739986..2743377)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (2739986..2743377)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These results reveal that ESCRT-0 (ESCRT-0 components stam and hrs)mutants inhibit EGFR signaling by disrupting Rhomboid endosomal trafficking in the ligand-producing cells.
Title: ESCRT-0 complex modulates Rbf-mutant cell survival by regulating Rhomboid endosomal trafficking and EGFR signaling.
The present report is devoted to the clarification and detailing of the Hrs effect on D/V, as well as on A/P borders of the imaginal wing disc compartments in Drosophila melanogaster.
Title: [hrs Gene and Borders of Compartments of Imaginal Wing Disc in Drosophila melanogaster].
Hrs is dispensable for cytokinesis. Finally, it was found that although Drosophila Hrs does not localize at acrosome, the other endosomal markers--Rab4, Rab7, and Rab11--are detected at the organelle.
Title: [COMPARATIVE STUDY OF Hrs AND OTHER ENDOSOMAL MARKERS CELLULAR LOCALIZATION IN DROSOPHILA MELANOGASTER SPERMATOGENESIS BY GFP-CHIMERICAL PROTEIN APPROACH].
Drosophila epithelia lacking Hrs and Stam, accumulate Notch and Dome in endosomes, maintain normal apico-basal polarity and proliferation control and do not show ectopic Notch signaling activation.
Title: ESCRT-0 is not required for ectopic Notch activation and tumor suppression in Drosophila.
Hrs mediates Smo trafficking in the late endosome by not only promoting Smo ubiquitination but also blocking Smo phosphorylation.
Title: Hrs promotes ubiquitination and mediates endosomal trafficking of smoothened in Drosophila hedgehog signaling.
these data support a model in which Ubpy plays a dual role in both cargo deubiquitylation and the ESCRT-0 subunit Hrs stability during development.
Title: Ubpy controls the stability of the ESCRT-0 subunit Hrs in development.
Hrs protein is involved in the formation of the D/V boundary of the wing imaginal disk and suggested a new role in wing vein refinement for the Hrs gene
Title: [Role of the Drosophila melanogaster hrs gene in wing formation].
show that the endosomal proteins Myopic (Mop) and Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) are required for the activation of the Toll signaling pathway.
Title: Endocytic pathway is required for Drosophila Toll innate immune signaling.
PtdIns(3)P specifically induces the membrane penetration of the FYVE domain of HRS protein and increases it membrane resonance time causing local conformational changes
Title: Phosphatidylinositol 3-phosphate induces the membrane penetration of the FYVE domains of Vps27p and Hrs.
intracellular accumulation of receptors in Hrs mutant cells could be due to defective sorting towards lysosomal degradation or to defective post-endocytic retention in endosomes
Title: Hrs mediates downregulation of multiple signalling receptors in Drosophila.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in early endosome to late endosome transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytic recycling	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosome transport via multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exosomal secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of torso signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron remodeling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of Toll signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Wnt protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor internalization	IBA Inferred from Biological aspect of Ancestor more info
involved_in receptor internalization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of epithelial cell migration, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in early endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: hepatocyte growth factor regulated tyrosine kinase substrate

Names: CG2903-PA; CG2903-PB; CG2903-PC; F[[1]]F[[0]] ATPase; HGF-regulated tyrosine kinase substrate; Hrs-PA; Hrs-PB; Hrs-PC; hepatocyte growth factor regulated tyrosine kinase; hepatocyte growth factor-regulated tyrosine kinase substrate; hepatocyte growth factor-regulated tyrosine substrate; hepatocyte growth-factor-regulated substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

2739986..2743377

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_164496.1 → NP_722830.2 hepatocyte growth factor regulated tyrosine kinase substrate, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_722830.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V018, Q8IGU6, Q960X8, Q9VQF2, Q9VQF3

UniProtKB/TrEMBL

B7FNJ7

Related

FBpp0088692, FBtr0089751

Conserved Domains (5) summary

cd03569
Location:2 → 143

VHS_Hrs_Vps27p; VHS domain family, Hrs and Vps27p subfamily; composed of Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and its yeast homolog Vps27p (vacuolar protein sorting). The VHS domain, an essential part of Hrs/Vps27p, has a superhelical ...

cd15720
Location:157 → 217

FYVE_Hrs; FYVE domain found in hepatocyte growth factor (HGF)-regulated tyrosine kinase substrate (Hrs) and similar proteins

pfam12210
Location:377 → 471

Hrs_helical; Hepatocyte growth factor-regulated tyrosine kinase substrate

cd16269
Location:501 → 512

GBP_C; coiled coil [structural motif]

cl20817
Location:456 → 520

GBP_C; Guanylate-binding protein, C-terminal domain
NM_080360.5 → NP_525099.3 hepatocyte growth factor regulated tyrosine kinase substrate, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_525099.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V018, Q8IGU6, Q960X8, Q9VQF2, Q9VQF3

UniProtKB/TrEMBL

B7FNJ7

Related

FBpp0088691

Conserved Domains (5) summary

cd03569
Location:2 → 143

VHS_Hrs_Vps27p; VHS domain family, Hrs and Vps27p subfamily; composed of Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and its yeast homolog Vps27p (vacuolar protein sorting). The VHS domain, an essential part of Hrs/Vps27p, has a superhelical ...

cd15720
Location:157 → 217

FYVE_Hrs; FYVE domain found in hepatocyte growth factor (HGF)-regulated tyrosine kinase substrate (Hrs) and similar proteins

pfam12210
Location:377 → 471

Hrs_helical; Hepatocyte growth factor-regulated tyrosine kinase substrate

cd16269
Location:501 → 512

GBP_C; coiled coil [structural motif]

cl20817
Location:456 → 520

GBP_C; Guanylate-binding protein, C-terminal domain
NM_164497.1 → NP_722831.1 hepatocyte growth factor regulated tyrosine kinase substrate, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_722831.1

Status: REVIEWED

UniProtKB/TrEMBL

C9QP23

Related

FBpp0088690, FBtr0089749

Conserved Domains (4) summary

cd15720
Location:44 → 104

FYVE_Hrs; FYVE domain found in hepatocyte growth factor (HGF)-regulated tyrosine kinase substrate (Hrs) and similar proteins

pfam12210
Location:264 → 358

Hrs_helical; Hepatocyte growth factor-regulated tyrosine kinase substrate

cd16269
Location:388 → 399

GBP_C; coiled coil [structural motif]

cl20817
Location:343 → 407

GBP_C; Guanylate-binding protein, C-terminal domain