Chrnb3 cholinergic receptor, nicotinic, beta polypeptide 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrnb3provided by MGI
Official Full Name: cholinergic receptor, nicotinic, beta polypeptide 3provided by MGI
Primary source: MGI:MGI:106212
See related: Ensembl:ENSMUSG00000031492 AllianceGenome:MGI:106212
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Acrb3; 5730417K16Rik
Summary: Predicted to enable acetylcholine binding activity and acetylcholine-gated monoatomic cation-selective channel activity. Predicted to contribute to acetylcholine receptor activity and heterocyclic compound binding activity. Involved in presynaptic modulation of chemical synaptic transmission. Is active in dopaminergic synapse. Is expressed in intestine; liver; nervous system; and retina. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; bipolar disorder; cocaine dependence; depressive disorder; and nicotine dependence. Orthologous to human CHRNB3 (cholinergic receptor nicotinic beta 3 subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Low expression observed in reference dataset See more
Orthologs: human all
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Genomic context

Location:: 8 A2; 8 16.0 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (27858688..27889758)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (27368711..27399730)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Beta3 nicotinic subunits do not seem to influence nicotine reward but plays an important role in affective nicotine withdrawal signs.
Title: The β3 subunit of the nicotinic acetylcholine receptor is required for nicotine withdrawal-induced affective but not physical signs or nicotine reward in mice.
Data provide evidence that the protective genetic variant at rs6474413 identified in human genetic studies reduces gene expression and that decreased beta3 gene expression in mice reduces nicotine intake
Title: The β3 subunit of the nicotinic acetylcholine receptor: Modulation of gene expression and nicotine consumption.
Chimeric nicotinic acetylcholine receptors reveal the roles for N-terminal extracellular domains of (nAChR) beta3 subunits in the receptor activity and function.
Title: Roles for N-terminal extracellular domains of nicotinic acetylcholine receptor (nAChR) β3 subunits in enhanced functional expression of mouse α6β2β3- and α6β4β3-nAChRs.
beta3 subunit coexpression promotes function of alpha6*-nAChR
Title: Modulation of gain-of-function α6*-nicotinic acetylcholine receptor by β3 subunits.
Data show that in Prkce(-/-) mice, conditioned place preference and self-administer behaviors are associated with reduced levels of alpha(6) and beta(3) nicotinic receptor subunit mRNA in the ventral midbrain and striatum.
Title: Protein kinase C epsilon modulates nicotine consumption and dopamine reward signals in the nucleus accumbens.
beta3-containing nicotinic receptors influence anxiety and may be critical for the continuation of smoking behaviors
Title: Decreased anxiety-like behavior in beta3 nicotinic receptor subunit knockout mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation

General gene information

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Markers

Chrnb3 (e-PCR), detects polymorphism
- UniSTS:256427

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylcholine binding	IEA Inferred from Electronic Annotation more info
enables acetylcholine binding	ISO Inferred from Sequence Orthology more info
contributes_to acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IEA Inferred from Electronic Annotation more info
enables heterocyclic compound binding	IEA Inferred from Electronic Annotation more info
contributes_to heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in neuromuscular synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in presynaptic modulation of chemical synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to nicotine	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, cholinergic	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IEA Inferred from Electronic Annotation more info
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
is_active_in dopaminergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in dopaminergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in dopaminergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit beta-3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_027454.4 → NP_081730.1 neuronal acetylcholine receptor subunit beta-3 isoform 2 precursor

See identical proteins and their annotated locations for NP_081730.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate segment, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

Source sequence(s)

AK017571, AK030464, BC058193

Consensus CDS

CCDS22216.1

UniProtKB/TrEMBL

Q3UZS0

Related

ENSMUSP00000078428.5, ENSMUST00000079463.12

Conserved Domains (3) summary

TIGR00860
Location:6 → 440

LIC; Cation transporter family protein

pfam02931
Location:35 → 224

Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain

pfam02932
Location:231 → 437

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region
NM_173212.4 → NP_775304.1 neuronal acetylcholine receptor subunit beta-3 isoform 1 precursor

See identical proteins and their annotated locations for NP_775304.1

Status: VALIDATED

Description

Transcript Variant: This variant represents the longer transcript and it encodes the longer protein (isoform 1).

Source sequence(s)

AK030464, AK133689, BC058193

Consensus CDS

CCDS22217.1

UniProtKB/Swiss-Prot

Q8BMN3, Q8R5H3, Q9CYK8

UniProtKB/TrEMBL

Q3UZS0

Related

ENSMUSP00000052297.4, ENSMUST00000060943.5

Conserved Domains (3) summary

TIGR00860
Location:6 → 455

LIC; Cation transporter family protein

pfam02931
Location:35 → 239

Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain

pfam02932
Location:246 → 452

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

27858688..27889758

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030243231.2 → XP_030099091.1 neuronal acetylcholine receptor subunit beta-3 isoform X1

Conserved Domains (1) summary

TIGR00860
Location:1 → 320

LIC; Cation transporter family protein