Capn2 calpain 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Capn2provided by MGI
Official Full Name: calpain 2provided by MGI
Primary source: MGI:MGI:88264
See related: Ensembl:ENSMUSG00000026509 AllianceGenome:MGI:88264
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Capa2; CALP80; Capa-2; m-calpin; m-calpain
Summary: Enables calcium-dependent cysteine-type endopeptidase activity. Involved in several processes, including cellular response to amino acid stimulus; protein catabolic process at postsynapse; and synaptic vesicle endocytosis. Acts upstream of or within blastocyst development and myoblast fusion. Located in several cellular components, including chromatin; cytosol; and lysosome. Is active in postsynapse. Is expressed in several structures, including central nervous system; lens epithelium; molar; oral region epithelium; and skin. Orthologous to human CAPN2 (calpain 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 35.3), subcutaneous fat pad adult (RPKM 29.4) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 H5; 1 84.93 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (182294818..182345175, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (182467253..182517610, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Targeting calpain-2-mediated junctophilin-2 cleavage delays heart failure progression following myocardial infarction.
Title: Targeting calpain-2-mediated junctophilin-2 cleavage delays heart failure progression following myocardial infarction.
The neuronal transcription factor MEIS2 is a calpain-2 protease target.
Title: The neuronal transcription factor MEIS2 is a calpain-2 protease target.
Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration.
Title: Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration.
Calpain-2 activation in mouse hippocampus plays a critical role in seizure-induced neuropathology.
Title: Calpain-2 activation in mouse hippocampus plays a critical role in seizure-induced neuropathology.
A new mouse model for retinal degeneration due to Fam161a deficiency.
Title: A new mouse model for retinal degeneration due to Fam161a deficiency.
Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice.
Title: Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice.
Inhibition of the ERK1/2-ubiquitous calpains pathway attenuates experimental pulmonary fibrosis in vivo and in vitro.
Title: Inhibition of the ERK1/2-ubiquitous calpains pathway attenuates experimental pulmonary fibrosis in vivo and in vitro.
Heat stress reduces calpain-2 expression, which then activates p38, leading to cardiomyocyte apoptosis and heart dysfunction.
Title: Calpain-2 protects against heat stress-induced cardiomyocyte apoptosis and heart dysfunction by blocking p38 mitogen-activated protein kinase activation.
Isoform-specific hyperactivation of calpain-2, but not calpain-1 occurs at the synapse early in the pathogenesis of Alzheimer's disease potentially contributing to the deregulation of synaptic signaling in humans and mice models.
Title: Isoform-specific hyperactivation of calpain-2 occurs presymptomatically at the synapse in Alzheimer's disease mice and correlates with memory deficits in human subjects.
Our data indicate that calpain-1 activity is neuroprotective and calpain-2 activity is neurodegenerative after traumatic Brain Injury
Title: Protection against TBI-Induced Neuronal Death with Post-Treatment with a Selective Calpain-2 Inhibitor in Mice.

Submit: New GeneRIF Correction See all GeneRIFs (63)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to calcium-dependent cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info
enables calcium-dependent cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	TAS Traceable Author Statement more info	PubMed
enables cytoskeletal protein binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal protein binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in behavioral response to pain	IEA Inferred from Electronic Annotation more info
involved_in behavioral response to pain	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within blastocyst development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to amino acid stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to amino acid stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to interferon-beta	IEA Inferred from Electronic Annotation more info
involved_in cellular response to lipopolysaccharide	IEA Inferred from Electronic Annotation more info
involved_in female pregnancy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within myoblast fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cardiac muscle cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of myoblast fusion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of myoblast fusion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphatidylcholine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of phosphatidylcholine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in protein autoprocessing	IEA Inferred from Electronic Annotation more info
involved_in protein autoprocessing	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process at postsynapse	EXP Inferred from Experiment more info	PubMed
involved_in protein catabolic process at postsynapse	IDA Inferred from Direct Assay more info	PubMed
involved_in protein catabolic process at postsynapse	IEP Inferred from Expression Pattern more info	PubMed
involved_in protein catabolic process at postsynapse	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	NAS Non-traceable Author Statement more info	PubMed
involved_in proteolysis involved in protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proteolysis involved in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of interleukin-6 production	IEA Inferred from Electronic Annotation more info
involved_in regulation of interleukin-6 production	ISO Inferred from Sequence Orthology more info
involved_in response to hydrogen peroxide	IEA Inferred from Electronic Annotation more info
involved_in response to hypoxia	IEA Inferred from Electronic Annotation more info
involved_in response to mechanical stimulus	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle endocytosis	EXP Inferred from Experiment more info	PubMed
involved_in synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle endocytosis	IEP Inferred from Expression Pattern more info	PubMed
involved_in synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
part_of calpain complex	ISO Inferred from Sequence Orthology more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in dendrite	IEA Inferred from Electronic Annotation more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in lysosome	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with membrane raft	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
colocalizes_with membrane raft	ISS Inferred from Sequence or Structural Similarity more info
located_in neuronal cell body	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in perinuclear endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info
located_in pseudopodium	IEA Inferred from Electronic Annotation more info
located_in pseudopodium	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: calpain-2 catalytic subunit

Names: 80 kDa M-calpain subunit; CANP 2; calcium-activated neutral proteinase 2; calpain M-type; calpain-2 large subunit; cysteine proteinase; m-calpain 80 kDa large subunit; m-calpain large subunit; millimolar-calpain

NP_033924.2

EC 3.4.22.53

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009794.4 → NP_033924.2 calpain-2 catalytic subunit

See identical proteins and their annotated locations for NP_033924.2

Status: VALIDATED

Source sequence(s)

AC131742, BC054726, BG091141

Consensus CDS

CCDS35813.1

UniProtKB/Swiss-Prot

O08529, O35518, O54843

UniProtKB/TrEMBL

Q3U3A8

Related

ENSMUSP00000068895.9, ENSMUST00000068505.10

Conserved Domains (4) summary

COG5126
Location:604 → 690

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

cd00051
Location:577 → 631

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00648
Location:46 → 342

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:356 → 507

Calpain_III; Calpain large subunit, domain III