aop anterior open [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: aopprovided by FlyBase
Official Full Name: anterior openprovided by FlyBase
Primary source: FLYBASE:FBgn0000097
Locus tag: Dmel_CG3166
See related: AllianceGenome:FB:FBgn0000097
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Aop; AOP; aop/yan; Aop/Yan; CG3166; Dmel\CG3166; DROYANET; DROYANETSB; fus6; pok; SK2-1; yan; Yan; YAN; yan/pok
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and protein domain specific binding activity. Involved in several processes, including cell surface receptor protein tyrosine kinase signaling pathway; negative regulation of cell differentiation; and regulation of gene expression. Located in cytosol and nucleus. Is expressed in several structures, including embryonic gnathal segment; germ layer; imaginal disc; larval optic anlage; and synaptic neuropil domain. Human ortholog(s) of this gene implicated in hematologic cancer (multiple) and thrombocytopenia. Orthologous to human ETV6 (ETS variant transcription factor 6) and ETV7 (ETS variant transcription factor 7). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 22D1-22D1; 2-5 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (2156484..2178754, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (2156484..2178754, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In Drosophila, reduced insulin/IGF signaling extends lifespan by coordinating activation of Aop and Foxo. Aop and Foxo bind the same genomic loci, and individually, they affect similar transcriptional programs in vivo. In combination, Aop can both moderate or synergise with Foxo, dependent on promoter context. Also, Foxo and Aop oppose the gene-regulatory activity of Pnt.
Title: Longevity is determined by ETS transcription factors in multiple tissues and diverse species.
a spectacular range of affinities lies latent within the SAM domain of Yan, and that these affinities must be tuned to preserve transcriptional repression and biological function during development.
Title: Tuned polymerization of the transcription factor Yan limits off-DNA sequestration to confer context-specific repression.
a new layer of regulation whereby Yan and Pointed co-occupy regulatory elements to repress gene expression in a coordinated manner, with Pointed being unexpectedly required for the genome-wide occupancy of both Yan and the co-repressor Groucho.
Title: Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila.
Yan recruitment promoted by this high-affinity ETS supersite not only biases Yan-Pnt competition at the specific CRM but also organizes Yan-repressive complexes in three dimensions across the eve locus
Title: Cooperative recruitment of Yan via a high-affinity ETS supersite organizes repression to confer specificity and robustness to cardiac cell fate specification.
DNA Occupancy of Polymerizing Transcription Factors
Title: DNA Occupancy of Polymerizing Transcription Factors: A Chemical Model of the ETS Family Factor Yan.
We show that acal lies downstream of raw, a pioneer protein and interacts genetically with two genes involved in JNK signaling and dorsal closure: Connector of kinase to AP1 (Cka) and anterior open.
Title: acal is a long non-coding RNA in JNK signaling in epithelial shape changes during drosophila dorsal closure.
Study identifies the E-twenty six (ETS) transcriptional repressor, Anterior open (Aop), as central to lifespan extension caused by reduced insulin/IGF-1 or Ras attenuation.
Title: The Ras-Erk-ETS-Signaling Pathway Is a Drug Target for Longevity.
instances of co-expressed Yan and Pnt-GFP in tissues with high RTK signaling cannot be explained by the current model, and thus they provide important contexts for future investigation of how context-specific differences in RTK signaling
Title: A comparative study of Pointed and Yan expression reveals new complexity to the transcriptional networks downstream of receptor tyrosine kinase signaling.
Anterior open inhibits MAP kinase and Wingless signaling to couple tracheal cell fate with branch identity.
Title: The ETS domain transcriptional repressor Anterior open inhibits MAP kinase and Wingless signaling to couple tracheal cell fate with branch identity.
a low level of Notch signaling functions to maintain the neuroepithelial cell identity by suppressing the expression of pointedP1 gene through the transcriptional repressor Anterior open.
Title: Changes in Notch signaling coordinates maintenance and differentiation of the Drosophila larval optic lobe neuroepithelia.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in R3/R4 cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of adult lifespan	IGI Inferred from Genetic Interaction more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure	TAS Traceable Author Statement more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermal growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fibroblast growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fibroblast growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in muscle cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of R7 cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of R7 cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of compound eye photoreceptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of fusion cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of terminal cell fate specification, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oenocyte delamination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of establishment of planar polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in sevenless signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in sevenless signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sevenless signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vascular endothelial growth factor receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: anterior open

Names: CG3166-PA; CG3166-PB; CG3166-PC; CG3166-PD; CG3166-PE; SGP cluster fusion defects 6; anterior-open; aop-PA; aop-PB; aop-PC; aop-PD; aop-PE; pokkuri; yan

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

2156484..2178754 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078731.3 → NP_523455.2 anterior open, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523455.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q01842, Q6AWH6, Q6NR43, Q9VQ81

UniProtKB/TrEMBL

M9PC88

Related

FBpp0077522

Conserved Domains (2) summary

cd08535
Location:49 → 116

SAM_PNT-Tel_Yan; Sterile alpha motif (SAM)/Pointed domain of Tel/Yan protein

smart00413
Location:395 → 482

ETS; erythroblast transformation specific domain
NM_001272979.1 → NP_001259908.1 anterior open, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259908.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q01842, Q6AWH6, Q6NR43, Q9VQ81

UniProtKB/TrEMBL

M9PC88

Conserved Domains (2) summary

cd08535
Location:49 → 116

SAM_PNT-Tel_Yan; Sterile alpha motif (SAM)/Pointed domain of Tel/Yan protein

smart00413
Location:395 → 482

ETS; erythroblast transformation specific domain
NM_164457.2 → NP_722766.1 anterior open, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_722766.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q01842, Q6AWH6, Q6NR43, Q9VQ81

UniProtKB/TrEMBL

M9PC88

Related

FBpp0077523

Conserved Domains (2) summary

cd08535
Location:49 → 116

SAM_PNT-Tel_Yan; Sterile alpha motif (SAM)/Pointed domain of Tel/Yan protein

smart00413
Location:395 → 482

ETS; erythroblast transformation specific domain
NM_001272980.1 → NP_001259909.1 anterior open, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259909.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q01842, Q6AWH6, Q6NR43, Q9VQ81

UniProtKB/TrEMBL

M9PC88

Conserved Domains (2) summary

cd08535
Location:49 → 116

SAM_PNT-Tel_Yan; Sterile alpha motif (SAM)/Pointed domain of Tel/Yan protein

smart00413
Location:395 → 482

ETS; erythroblast transformation specific domain
NM_001272981.1 → NP_001259910.1 anterior open, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259910.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q01842, Q6AWH6, Q6NR43, Q9VQ81

UniProtKB/TrEMBL

M9PC88

Conserved Domains (2) summary

cd08535
Location:49 → 116

SAM_PNT-Tel_Yan; Sterile alpha motif (SAM)/Pointed domain of Tel/Yan protein

smart00413
Location:395 → 482

ETS; erythroblast transformation specific domain