msps mini spindles [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: mspsprovided by FlyBase
Official Full Name: mini spindlesprovided by FlyBase
Primary source: FLYBASE:FBgn0027948
Locus tag: Dmel_CG5000
See related: AllianceGenome:FB:FBgn0027948
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0140519.136; CG5000; Dis1; Dmel\CG5000; Msps; MSPS; TOG; XMAP215
Summary: Enables tubulin binding activity. Involved in several processes, including bicoid mRNA localization; generation of neurons; and microtubule cytoskeleton organization. Located in centrosome; microtubule plus-end; and spindle. Is expressed in several structures, including CNS glial cell; anlage in statu nascendi; ganglia; germline cell; and head epidermis primordium. Orthologous to human CKAP5 (cytoskeleton associated protein 5). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 89B1-89B2; 3-58 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (16050801..16060675)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (11876523..11886397)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The dynamic duo of microtubule polymerase Mini spindles/XMAP215 and cytoplasmic dynein is essential for maintaining Drosophila oocyte fate.
Title: The dynamic duo of microtubule polymerase Mini spindles/XMAP215 and cytoplasmic dynein is essential for maintaining Drosophila oocyte fate.
A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila.
Title: A microtubule polymerase is required for microtubule orientation and dendrite pruning in Drosophila.
Msps tubulin overexpressed gene domains have distinct architectures that bind either free or polymerized tubulin, and that a polarized array drives microtubule polymerization.
Title: TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation.
Findings suggest that Msps is a novel Rac effector that acts, in partnership with Orbit, to regionally regulate microtubule dynamics.
Title: TOG Proteins Are Spatially Regulated by Rac-GSK3β to Control Interphase Microtubule Dynamics.
Drosophila melanogaster mini spindles TOG3 utilizes unique structural elements to promote domain stability and maintain a TOG1- and TOG2-like tubulin-binding surface.
Title: Drosophila melanogaster mini spindles TOG3 utilizes unique structural elements to promote domain stability and maintain a TOG1- and TOG2-like tubulin-binding surface.
MT dynamics are promoted by the independent as well as the cooperative action of XMAP215(msps) polymerase and the EB1-Sentin duo.
Title: Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin.
Msps exhibits EB1-dependent and spatially regulated microtubule localization, targeting to microtubule plus ends in the cell interior and decorating the lattice of growing and shrinking microtubules in the cell periphery
Title: The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics.
Data suggest a model in which CLASP and Msps converge in an antagonistic balance in the Abl signaling pathway.
Title: Parallel genetic and proteomic screens identify Msps as a CLASP-Abl pathway interactor in Drosophila.
The morphology of Exu particles is msps-dependent, and Exu is specifically associated with tubular endoplasmic reticulum in msps mutants.
Title: Microtubules, the ER and Exu: new associations revealed by analysis of mini spindles mutations.
Cnn Motif 1 is necessary for proper recruitment of gamma-tubulin, D-TACC (the homolog of vertebrate transforming acidic coiled-coil proteins [TACC]), and Minispindles (Msps) to embryonic centrosomes
Title: Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule plus end polymerase	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule plus end polymerase	IEA Inferred from Electronic Annotation more info
enables microtubule plus-end binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables tubulin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in bicoid mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome duplication	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoplasmic microtubule organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IEA Inferred from Electronic Annotation more info
involved_in establishment or maintenance of neuroblast polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule polymerization	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	HMP more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in oocyte microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of microtubule nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in pronuclear fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle organization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	TAS Traceable Author Statement more info	PubMed
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule plus-end	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: mini spindles

Names: CG5000-PB; CG5000-PC; CG5000-PD; CG5000-PE; CG5000-PF; mini-spindles; minispindles; msps-PB; msps-PC; msps-PD; msps-PE; msps-PF

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

16050801..16060675

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001260200.2 → NP_001247129.1 mini spindles, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247129.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K700, Q9U5W6

Conserved Domains (1) summary

sd00044
Location:860 → 891

HEAT; HEAT repeat [structural motif]
NM_001260201.2 → NP_001247130.1 mini spindles, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247130.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K664, Q9U5W6

Conserved Domains (1) summary

sd00044
Location:860 → 891

HEAT; HEAT repeat [structural motif]
NM_001170156.2 → NP_001163627.1 mini spindles, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163627.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JIM4, Q9U5W6

Conserved Domains (1) summary

sd00044
Location:860 → 891

HEAT; HEAT repeat [structural motif]
NM_169698.3 → NP_732105.2 mini spindles, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_732105.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q4QQC0, Q9VEZ3

UniProtKB/TrEMBL

Q9U5W6

Conserved Domains (1) summary

sd00044
Location:860 → 891

HEAT; HEAT repeat [structural motif]
NM_001260202.1 → NP_001247131.1 mini spindles, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247131.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JIM4, Q9U5W6

Conserved Domains (1) summary

sd00044
Location:860 → 891

HEAT; HEAT repeat [structural motif]