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    VCX1 Vcx1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851429, updated on 9-Dec-2024

    Summary

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    Official Symbol
    VCX1
    Official Full Name
    Vcx1p
    Primary source
    SGD:S000002286
    Locus tag
    YDL128W
    See related
    AllianceGenome:SGD:S000002286; FungiDB:YDL128W; VEuPathDB:YDL128W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    HUM1; MNR1
    Summary
    Enables calcium:proton antiporter activity and potassium:proton antiporter activity. Involved in calcium ion transport; intracellular calcium ion homeostasis; and transmembrane transport. Located in fungal-type vacuole membrane. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See VCX1 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (232652..233887)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene ribosomal protein P1B Neighboring gene uncharacterized protein Neighboring gene cyclin PCL2 Neighboring gene AAA family ATPase CDC48

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A yeast expression system for functional and pharmacological studies of the malaria parasite Ca²⁺/H⁺ antiporter. Salcedo-Sora JE, et al. Malar J, 2012 Aug 1. PMID 22853777, Free PMC Article
    2. Structural basis for alternating access of a eukaryotic calcium/proton exchanger. Waight AB, et al. Nature, 2013 Jul 4. PMID 23685453, Free PMC Article
    3. Mutants resistant to manganese in Saccharomyces cerevisiae. Bianchi ME, et al. Curr Genet, 1981 Dec. PMID 24185995
    4. A single nucleotide change in the MNR1 (VCX1/HUM1) gene determines resistance to manganese in Saccharomyces cerevisiae. del Pozo L, et al. Yeast, 1999 Mar 30. PMID 10219995
    5. Topology mapping of the vacuolar Vcx1p Ca2+/H+ exchanger from Saccharomyces cerevisiae. Segarra VA, et al. Biochem J, 2008 Aug 15. PMID 18447831

    See all (62) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Importantly, ESCRT gene deletions in the vcx1Delta strain did not result in an increase in the CaCl2-invoked drop in the intracellular pH levels of cells, which demonstrates a genetic interaction between VCX1 and studied ESCRT genes.
      Title: Vcx1 and ESCRT components regulate intracellular pH homeostasis in the response of yeast cells to calcium stress.
    2. crystal structure of the Saccharomyces cerevisiae vacuolar Ca(2+)/H(+) exchanger (Vcx1) at 2.3 A resolution in a cytosol-facing, substrate-bound conformation
      Title: Structural basis for alternating access of a eukaryotic calcium/proton exchanger.
    3. analysis of the relative importance of Nhx1p, Vnx1p, and Vcx1p in the vacuolar accumulation of monovalent cations
      Title: Vacuolar cation/H+ antiporters of Saccharomyces cerevisiae.
    4. Vcx1 protein has an odd number of transmembrane domains; its termini are located on opposite sides of the membrane, with the N-terminus in the cytoplasm.
      Title: Topology mapping of the vacuolar Vcx1p Ca2+/H+ exchanger from Saccharomyces cerevisiae.
    5. Results demonstrate that calcineurin can positively regulate the transport of cations by the mutant allele VCX1-M1p.
      Title: Functional dependence on calcineurin by variants of the Saccharomyces cerevisiae vacuolar Ca2+/H+ exchanger Vcx1p.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables calcium:proton antiporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables calcium:proton antiporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables calcium:proton antiporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables calcium:proton antiporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables potassium:proton antiporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in calcium ion transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in calcium ion transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in calcium ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in calcium ion transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular calcium ion homeostasis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular calcium ion homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in potassium ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proton transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in fungal-type vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in fungal-type vacuole membrane HDA PubMed 
    is_active_in fungal-type vacuole membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in fungal-type vacuole membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuolar membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Vcx1p
    NP_010155.1
    • Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      232652..233887
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180187.1 → NP_010155.1  TPA: Vcx1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010155.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VRM2, O13580, O13581, Q99385
      UniProtKB/TrEMBL
      G2WC34, N1P5J6
      Conserved Domains (1) summary
      TIGR00378
      Location:43 → 396
      cax; calcium/proton exchanger (cax)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q99385.1

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