KAP95 karyopherin beta [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: KAP95
Official Full Name: karyopherin beta
Primary source: SGD:S000004339
Locus tag: YLR347C
See related: AllianceGenome:SGD:S000004339; FungiDB:YLR347C; VEuPathDB:YLR347C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RSL1
Summary: Enables guanyl-nucleotide exchange factor activity and nuclear import signal receptor activity. Involved in several processes, including establishment of protein localization; nuclear pore complex assembly; and regulation of protein desumoylation. Located in cytoplasm and nucleus. Part of NLS-dependent protein nuclear import complex. Orthologous to human KPNB1 (karyopherin subunit beta 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See KAP95 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (823828..826413, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A designer FG-Nup that reconstitutes the selective transport barrier of the nuclear pore complex.
Title: A designer FG-Nup that reconstitutes the selective transport barrier of the nuclear pore complex.
Data suggest that, in nuclear exosomes, karyopherins are bound to the Rrp6 complex; Srp1, Kap95, and Sxm1 appear to be the most important karyopherins for nuclear import of Rrp6. (Rrp6 = exosome nuclease subunit RRP6; Srp1 = karyopherin alpha kap60; Kap95 = karyopherin beta Kap95; Sxm1 = karyopherin Kap108)
Title: Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in Saccharomyces cerevisiae.
The Kap95 is able to mediate efficient Mig2-Kap95 interaction in the absence of the importin-alpha.
Title: Yeast importin-β is required for nuclear import of the Mig2 repressor.
Hxk2 as a new cargo for the alpha/beta-importin pathway of S. cerevisiae.
Title: Nuclear import of the yeast hexokinase 2 protein requires α/β-importin-dependent pathway.
Kap95 is likely the primary karyopherin responsible for Replication protein A nuclear import in yeast.
Title: The karyopherin Kap95 and the C-termini of Rfa1, Rfa2, and Rfa3 are necessary for efficient nuclear import of functional RPA complex proteins in Saccharomyces cerevisiae.
The authors identified karyopherin Kap95 as the transport factor responsible for the nuclear import of Slt2 and Rlm1.
Title: Yeast karyopherins Kap123 and Kap95 are related to the function of the cell integrity pathway.
Pct1 interacts with Kap60 and Kap95 with this tripartite complex transiting the nuclear pore.
Title: The Kap60-Kap95 karyopherin complex directly regulates phosphatidylcholine synthesis.
Data show the crystal structure of the Kap95p-RanGDP complex shows that Kap95p induces the switch I and II regions of RanGDP to adopt a conformation that resembles that of the GTP-bound form.
Title: Kap95p binding induces the switch loops of RanGDP to adopt the GTP-bound conformation: implications for nuclear import complex assembly dynamics.
identification of a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p
Title: Structural basis for nuclear import complex dissociation by RanGTP.
Kap95 serves as a key regulator of nuclear pore complex assembly into intact nuclear envelopes.
Title: The karyopherin Kap95 regulates nuclear pore complex assembly into intact nuclear envelopes in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables nuclear import signal receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables nuclear import signal receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables nuclear localization sequence binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in NLS-bearing protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in import into nucleus	IEA Inferred from Electronic Annotation more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in nuclear pore complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into nucleus	IEA Inferred from Electronic Annotation more info
involved_in protein targeting to membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of nucleocytoplasmic transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein desumoylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of NLS-dependent protein nuclear import complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear pore	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: karyopherin beta

NP_013451.1

Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

823828..826413 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182236.1 → NP_013451.1 TPA: karyopherin beta [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013451.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYY6, Q06142

UniProtKB/TrEMBL

A7A1M6, B3RHL7, C7GRX3, C8ZDV4, G2WJG6, N1P1N7

Conserved Domains (2) summary

COG5215
Location:1 → 861

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

sd00044
Location:326 → 357

HEAT; HEAT repeat [structural motif]