gcs-1 Glutamate--cysteine ligase [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: gcs-1
Official Full Name: Glutamate--cysteine ligase
Primary source: WormBase:WBGene00001527
Locus tag: CELE_F37B12.2
See related: AllianceGenome:WB:WBGene00001527
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable glutamate-cysteine ligase activity. Involved in several processes, including glutathione biosynthetic process; response to arsenic-containing substance; and response to superoxide. Predicted to be part of glutamate-cysteine ligase complex. Is expressed in ASIL; ASIR; intestine; and pharynx. Human ortholog(s) of this gene implicated in cystic fibrosis; glutamate-cysteine ligase deficiency; hemolytic anemia; and myocardial infarction. Orthologous to human GCLC (glutamate-cysteine ligase catalytic subunit). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See gcs-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 7

Sequence:: Chromosome: II; NC_003280.10 (9035562..9038301, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

only 1mM DEM caused upregulation of egl-1, a gene controlled by a p53-orthologue, CEP-1.we suppressed GSH biosynthesis in worms by attenuating g-glutamylcysteine synthetase (gcs-1) expression through RNAi.gcs-1 knockdown commencing right after hatching impaired nematode stress resistance and rendered young adult worms prone to vulval ruptures during egg-laying.
Title: Non-linear impact of glutathione depletion on C. elegans life span and stress resistance.
PMK-1 phosphorylates SKN-1, leading to its accumulation in intestine nuclei, where SKN-1 activates transcription of gcs-1.
Title: The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F37B12.2 (e-PCR)
- UniSTS:307613

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables glutamate-cysteine ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamate-cysteine ligase activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in glutathione biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutathione biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in glutathione biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to arsenic-containing substance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to heat	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to reactive oxygen species	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to reactive oxygen species	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to superoxide	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
part_of glutamate-cysteine ligase complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Glutamate--cysteine ligase

NP_495927.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

9035562..9038301 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_063526.9 → NP_495927.2 Glutamate--cysteine ligase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495927.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q20117

Conserved Domains (1) summary

pfam03074
Location:241 → 612

GCS; Glutamate-cysteine ligase