tbc-2 PH domain-containing protein [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: tbc-2
Official Full Name: PH domain-containing protein
Primary source: WormBase:WBGene00022880
Locus tag: CELE_ZK1248.10
See related: AllianceGenome:WB:WBGene00022880
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables GTPase activator activity. Involved in positive regulation of early endosome to late endosome transport and regulation of presynaptic dense core granule exocytosis. Located in cholinergic synapse and late endosome. Is expressed in gonadal sheath cell; hypodermis; intestine; and pharyngeal muscle cell. Orthologous to several human genes including TBC1D2 (TBC1 domain family member 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See tbc-2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 11

Sequence:: Chromosome: II; NC_003280.10 (5823856..5827443, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Rab GTPase activating protein TBC-2 regulates endosomal localization of DAF-16 FOXO and lifespan.
Title: The Rab GTPase activating protein TBC-2 regulates endosomal localization of DAF-16 FOXO and lifespan.
VPS-34 recruits TBC-2 to endosomes to inactivate RAB-5 to ensure the directionality of endosome maturation
Title: The VPS34 PI3K negatively regulates RAB-5 during endosome maturation.
the loss of TBC-2 or AMPH-1 leads to abnormally high spatial overlap of RAB-5 and RAB-10. RAB-10 and AMPH-1 mediated down-regulation of RAB-5 is an important step in recycling, required for cargo exit from early endosomes
Title: Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes.
down-regulation of early endosome regulator RAB-5/Rab5 by a CED-5, CED-12, CED-10, TBC-2 cascade is an important step in the transport of cargo through the basolateral recycling endosome for delivery to the plasma membrane.
Title: CED-10/Rac1 regulates endocytic recycling through the RAB-5 GAP TBC-2.
TBC-2 is required for yolk protein storage during embryonic development; yolk constitutes an important energy source for larval survival during L1 diapause
Title: TBC-2 is required for embryonic yolk protein storage and larval survival during L1 diapause in Caenorhabditis elegans.
Data suggest that TBC-2 functions on RAB-7-positive late endosomes to inactivate RAB-5 during endosome maturation.
Title: TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in Caenorhabditis elegans.
tbc-2 encodes a Rab GTPase activating protein (GAP) and its loss of function affects several events of phagosome maturation
Title: C. elegans Rab GTPase activating protein TBC-2 promotes cell corpse degradation by regulating the small GTPase RAB-5.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ZK1248.10 (e-PCR)
- UniSTS:310429

ZK1248.11 (e-PCR)
- UniSTS:320676

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in positive regulation of early endosome to late endosome transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of presynaptic dense core granule exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of presynaptic dense core granule exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in cholinergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in presynapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: PH domain-containing protein

NP_495156.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

5823856..5827443 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_062755.9 → NP_495156.1 PH domain-containing protein [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495156.1

Status: REVIEWED

UniProtKB/TrEMBL

Q23419

Conserved Domains (5) summary

smart00164
Location:627 → 849

TBC; Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs

cd01265
Location:20 → 119

PH_TBC1D2A; TBC1 domain family member 2A pleckstrin homology (PH) domain

pfam00169
Location:20 → 111

PH; PH domain

pfam07889
Location:289 → 368

DUF1664; Protein of unknown function (DUF1664)

pfam08614
Location:235 → 400

ATG16; Autophagy protein 16 (ATG16)