nth-1 Endonuclease III homolog [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: nth-1
Official Full Name: Endonuclease III homolog
Primary source: WormBase:WBGene00011201
Locus tag: CELE_R10E4.5
See related: AllianceGenome:WB:WBGene00011201
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables 5-formyluracil DNA N-glycosylase activity and 5-hydroxymethyluracil DNA N-glycosylase activity. Involved in cellular response to hydrogen peroxide and depyrimidination. Predicted to be located in mitochondrion. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in familial adenomatous polyposis 3. Orthologous to human NTHL1 (nth like DNA glycosylase 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See nth-1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 6

Sequence:: Chromosome: III; NC_003281.10 (4291543..4294253, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

we could detect no deficiency in base excision repair in the nth-1 strain.
Title: In vivo repair of alkylating and oxidative DNA damage in the mitochondrial and nuclear genomes of wild-type and glycosylase-deficient Caenorhabditis elegans.
Loss of NTH-1, the only Base Excision Repair enzyme known to initiate repair of oxidative DNA damage in C. elegans, restores normal lifespan of the short-lived Nucleotide Excision Repair deficient xpa-1 mutant.
Title: A two-tiered compensatory response to loss of DNA repair modulates aging and stress response pathways.
Data show that the protein encoded by the sequence containing both R10E4.5 and the 117-bp region upstream from it, and found that the protein had the NTH activity.
Title: Purification and characterization of Caenorhabditis elegans NTH, a homolog of human endonuclease III: essential role of N-terminal region.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

R10E4.6 (e-PCR)
- UniSTS:321637

R10E4.5 (e-PCR)
- UniSTS:314787

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables 5-formyluracil DNA N-glycosylase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5-hydroxymethyluracil DNA N-glycosylase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA N-glycosylase activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on glycosyl bonds	IEA Inferred from Electronic Annotation more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity	IDA Inferred from Direct Assay more info	PubMed
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in base-excision repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair, AP site formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in base-excision repair, AP site formation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to hydrogen peroxide	IGI Inferred from Genetic Interaction more info	PubMed
involved_in depyrimidination	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleotide-excision repair	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info	PubMed

General protein information

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Preferred Names: Endonuclease III homolog

NP_001254907.1

Confirmed by transcript evidence

NP_001254908.1

Confirmed by transcript evidence

NP_001254909.1

Confirmed by transcript evidence

NP_001369882.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

4291543..4294253 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001267978.2 → NP_001254907.1 Endonuclease III homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001254907.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P54137

Conserved Domains (3) summary

smart00525
Location:221 → 239

FES; iron-sulpphur binding domain in DNA-(apurinic or apyrimidinic site) lyase (subfamily of ENDO3)

smart00478
Location:71 → 219

ENDO3c; endonuclease III

COG0177
Location:34 → 239

Nth; Endonuclease III [Replication, recombination and repair]
NM_001384068.1 → NP_001369882.1 Endonuclease III homolog [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/Swiss-Prot

C7G4V3, D5MCR0, D5MCR1, D5MCR2, P54137

Conserved Domains (1) summary

COG0177
Location:39 → 244

Nth; Endonuclease III [Replication, recombination and repair]
NM_001267979.3 → NP_001254908.1 Endonuclease III homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001254908.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P54137

Conserved Domains (3) summary

smart00525
Location:152 → 170

FES; iron-sulpphur binding domain in DNA-(apurinic or apyrimidinic site) lyase (subfamily of ENDO3)

smart00478
Location:2 → 150

ENDO3c; endonuclease III

COG0177
Location:1 → 170

Nth; Endonuclease III [Replication, recombination and repair]
NM_001267980.3 → NP_001254909.1 Endonuclease III homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001254909.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P54137

Conserved Domains (2) summary

smart00525
Location:68 → 86

FES; iron-sulpphur binding domain in DNA-(apurinic or apyrimidinic site) lyase (subfamily of ENDO3)

cl23768
Location:2 → 75

ENDO3c; endonuclease III; includes endonuclease III (DNA-(apurinic or apyrimidinic site) lyase), alkylbase DNA glycosidases (Alka-family) and other DNA glycosidases