Mkp3 Mitogen-activated protein kinase phosphatase 3 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Mkp3provided by FlyBase
Official Full Name: Mitogen-activated protein kinase phosphatase 3provided by FlyBase
Primary source: FLYBASE:FBgn0036844
Locus tag: Dmel_CG14080
See related: AllianceGenome:FB:FBgn0036844
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG14080; CG14081; Dmel\CG14080; DMKP-3; dMKP3; Dmkp3; DMKP3; mkp-3; mkp3; MKP3
Summary: Enables MAP kinase tyrosine phosphatase activity. Involved in several processes, including imaginal disc-derived wing vein specification; negative regulation of signal transduction; and open tracheal system development. Located in cytosol. Is expressed in several structures, including anlage in statu nascendi; endoderm anlage; gut section; muscle cell; and presumptive wing vein. Human ortholog(s) of this gene implicated in hypogonadotropic hypogonadism 19 with or without anosmia. Orthologous to human DUSP6 (dual specificity phosphatase 6); DUSP7 (dual specificity phosphatase 7); and DUSP9 (dual specificity phosphatase 9). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 75F6-75F6; 3-45 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (19066155..19086426, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (19059255..19079526, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We show that Tay bridge antagonizes EGFR signalling in the Drosophila melanogaster wing disc and other tissues, and that the protein interacts with both Erk and Mkp3
Title: Tay bridge is a negative regulator of EGFR signalling and interacts with Erk and Mkp3 in the Drosophila melanogaster wing.
Study suggests that Mkp3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway.
Title: Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3.
MKP-3 is critically involved in cell differentiation, proliferation, and gene expression by suppressing the Ras/ERK pathway, specifically binding to ERK via the N-terminal ERK-binding domain of MKP-3.
Title: MKP-3 has essential roles as a negative regulator of the Ras/mitogen-activated protein kinase pathway during Drosophila development.
Drosophila ERK specifically regulates DMKP-3 in terms of its enzyme activity and protein stability esssential for regulation.
Title: Regulation of Drosophila MKP-3 by Drosophila ERK.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm2290 (e-PCR)
- UniSTS:47936

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables MAP kinase tyrosine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase tyrosine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase tyrosine/serine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase tyrosine/serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in imaginal disc-derived wing vein specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of epithelial integrity, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mucosal immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of ERK1 and ERK2 cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of ERK1 and ERK2 cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of ERK1 and ERK2 cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of epidermal growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in primary branching, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein dephosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Mitogen-activated protein kinase phosphatase 3

Names: CG14080-PA; CG14080-PB; CG14080-PC; CG14080-PD; ERK-inhibitory phosphatase; MAP kinase phosphatase 3; MAPK phosphatase 3; MAPK phosphatase-3; MKP-3-like; MKP-like; Mkp3-PA; Mkp3-PB; Mkp3-PC; Mkp3-PD; drosophila MKP-3

NP_001262031.1

EC 3.1.3.-

NP_001262032.1

EC 3.1.3.-

NP_649087.1

EC 3.1.3.-

NP_730385.1

EC 3.1.3.-

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

19066155..19086426 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001275103.1 → NP_001262032.1 Mitogen-activated protein kinase phosphatase 3, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262032.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86P14, Q95SV1, Q9VVW4, Q9VVW5

UniProtKB/TrEMBL

M9PFW0

Conserved Domains (2) summary

cd01446
Location:8 → 148

DSP_MapKP; N-terminal regulatory rhodanese domain of dual specificity phosphatases (DSP), such as Mapk Phosphatase. This domain is believed to determine substrate specificity by binding the substrate, such as ERK2, and activating the C-terminal catalytic domain by ...

cd14566
Location:215 → 352

DSP_MKP_classII; dual specificity phosphatase domain of class II mitogen-activated protein kinase phosphatase
NM_001275102.1 → NP_001262031.1 Mitogen-activated protein kinase phosphatase 3, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262031.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PG13

Conserved Domains (3) summary

COG2453
Location:242 → 359

CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]

cd00127
Location:215 → 353

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

cd01446
Location:8 → 148

DSP_MapKP; N-terminal regulatory rhodanese domain of dual specificity phosphatases (DSP), such as Mapk Phosphatase. This domain is believed to determine substrate specificity by binding the substrate, such as ERK2, and activating the C-terminal catalytic domain by ...
NM_168785.1 → NP_730385.1 Mitogen-activated protein kinase phosphatase 3, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730385.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9VVW5

Related

FBpp0074803, FBtr0075036

Conserved Domains (2) summary

COG2453
Location:72 → 189

CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]

cd00127
Location:45 → 183

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...
NM_140830.2 → NP_649087.1 Mitogen-activated protein kinase phosphatase 3, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_649087.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86P14, Q95SV1, Q9VVW4, Q9VVW5

UniProtKB/TrEMBL

M9PFW0

Related

FBpp0074802

Conserved Domains (2) summary

cd01446
Location:8 → 148

DSP_MapKP; N-terminal regulatory rhodanese domain of dual specificity phosphatases (DSP), such as Mapk Phosphatase. This domain is believed to determine substrate specificity by binding the substrate, such as ERK2, and activating the C-terminal catalytic domain by ...

cd14566
Location:215 → 352

DSP_MKP_classII; dual specificity phosphatase domain of class II mitogen-activated protein kinase phosphatase