ISA2 Isa2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ISA2
Official Full Name: Isa2p
Primary source: SGD:S000006271
Locus tag: YPR067W
See related: AllianceGenome:SGD:S000006271; FungiDB:YPR067W; VEuPathDB:YPR067W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables iron ion binding activity. Involved in biotin biosynthetic process; iron-sulfur cluster assembly; and protein maturation by [4Fe-4S] cluster transfer. Located in mitochondrial intermembrane space and mitochondrial matrix. Human ortholog(s) of this gene implicated in multiple mitochondrial dysfunctions syndrome 4. Orthologous to human ISCA2 (iron-sulfur cluster assembly 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ISA2 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (682220..682777)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A novel substrate, Isa2, is likely processed by Icp55 in two consecutive steps and thus might represent the first example of a triple processing event in a mitochondrial precursor protein.
Title: Novel highly sensitive, specific, and straightforward strategy for comprehensive N-terminal proteomics reveals unknown substrates of the mitochondrial peptidase Icp55.
define Isa1, Isa2, and Iba57 as a specialized, late-acting ISC assembly subsystem that is specifically dedicated to the maturation of mitochondrial [4Fe-4S] proteins.
Title: Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 2 iron, 2 sulfur cluster binding	IBA Inferred from Biological aspect of Ancestor more info
enables 4 iron, 4 sulfur cluster binding	IBA Inferred from Biological aspect of Ancestor more info
enables iron ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables iron ion binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in biotin biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in biotin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in iron-sulfur cluster assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in iron-sulfur cluster assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in iron-sulfur cluster assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by [4Fe-4S] cluster transfer	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein maturation by [4Fe-4S] cluster transfer	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by iron-sulfur cluster transfer	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial intermembrane space	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Isa2p

NP_015392.1

Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

682220..682777

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184164.1 → NP_015392.1 TPA: Isa2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015392.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W471, Q12425

UniProtKB/TrEMBL

B3LLC0, B5VTL4, C7GY51, C8ZJ92, G2WPN9, N1NVK3

Conserved Domains (1) summary

COG0316
Location:58 → 183

IscA; Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones]