STE4 G protein subunit beta [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STE4
Official Full Name: G protein subunit beta
Primary source: SGD:S000005738
Locus tag: YOR212W
See related: AllianceGenome:SGD:S000005738; FungiDB:YOR212W; VEuPathDB:YOR212W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HMD2
Summary: Enables several functions, including G-protein alpha-subunit binding activity; G-protein gamma-subunit binding activity; and enzyme binding activity. Involved in several processes, including chemotropism; invasive growth in response to glucose limitation; and protein localization to mating projection tip. Located in mating projection and plasma membrane. Part of Cdc24p-Far1p-Gbetagamma complex; G-protein beta/gamma-subunit complex; and heterotrimeric G-protein complex. Human ortholog(s) of this gene implicated in several diseases, including Charcot-Marie-Tooth disease dominant intermediate F; artery disease (multiple); autosomal dominant intellectual developmental disorder 42; congenital stationary night blindness 1H; and hematologic cancer (multiple). Orthologous to several human genes including GNB2 (G protein subunit beta 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See STE4 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (742910..744181)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that the pheromone-responsive G protein subunits beta/gamma (Gbetagamma; STE4/STE18) dimer promotes the polarization of the pheromone receptor by interacting with casein kinases 1 or 2 (Yck1/2) and locally inhibiting receptor phosphorylation.
Title: Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.
The Dse1-Gbeta interaction antagonizes Gbetagamma signaling, including pheromone-activation of the invasive growth pathway.
Title: Dse1 may control cross talk between the pheromone and filamentation pathways in yeast.
These results indicate that chemoattractant-induced cell polarization requires continuous receptor-Galphabetagamma communication but not modulation of MAP kinase signaling.
Title: Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein.
KSS1 is activated by Ste4.
Title: Differential input by Ste5 scaffold and Msg5 phosphatase route a MAPK cascade to multiple outcomes.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables G-protein alpha-subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables G-protein gamma-subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables scaffold protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor complex adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables small GTPase binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in chemotropism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in invasive growth in response to glucose limitation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to mating projection tip	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone	IEA Inferred from Electronic Annotation more info
involved_in response to pheromone triggering conjugation with cellular fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in response to pheromone triggering conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone triggering conjugation with cellular fusion	IPI Inferred from Physical Interaction more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Cdc24p-Far1p-Gbetagamma complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cdc24p-Far1p-Gbetagamma complex	IPI Inferred from Physical Interaction more info	PubMed
part_of G-protein beta/gamma-subunit complex	IDA Inferred from Direct Assay more info	PubMed
part_of G-protein beta/gamma-subunit complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of G-protein beta/gamma-subunit complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IDA Inferred from Direct Assay more info	PubMed
part_of heterotrimeric G-protein complex	IMP Inferred from Mutant Phenotype more info	PubMed
located_in mating projection	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: G protein subunit beta

NP_014855.3

G protein beta subunit; forms a dimer with Ste18p to activate mating signaling pathway, forms heterotrimer with Gpa1p and Ste18p to dampen signaling; pheromone-induced phosphorylation plays critical role in chemotropism; may recruit Rho1p to polarized growth site during mating; contains WD40 repeats

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

742910..744181

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183631.3 → NP_014855.3 TPA: G protein subunit beta [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014855.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2R8, P18851

UniProtKB/TrEMBL

A6ZP55, B3LJN6, B5VS95, C7GWH0, C8ZGW7, G2WN84, N1NYN2

Conserved Domains (2) summary

cd00200
Location:87 → 419

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:95 → 132

7WD40; WD40 repeat [structural motif]