spn-A spindle A [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: spn-Aprovided by FlyBase
Official Full Name: spindle Aprovided by FlyBase
Primary source: FLYBASE:FBgn0003479
Locus tag: Dmel_CG7948
See related: AllianceGenome:FB:FBgn0003479
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG7948; D37788; Dm Rad51; Dmel\CG7948; DMR; DMR/DroRAD51; DMR1; dmrad51; dmRAD51; Dmrad51; DmRad51; DmRAD51; dRad51; RA51; RA51_DROME; rad51; Rad51; RAD51; Rad51dm; Spn-A; spnA; SpnA
Summary: Predicted to enable DNA binding activity and catalytic activity, acting on DNA. Involved in several processes, including oogenesis; regulation of double-strand break repair via homologous recombination; and specification of axis polarity. Predicted to be active in condensed nuclear chromosome. Is expressed in several structures, including central brain primordium; embryonic brain; embryonic/larval midgut; and embryonic/larval midgut primordium. Human ortholog(s) of this gene implicated in Fanconi anemia complementation group R; breast cancer; congenital mirror movement disorder; and ovarian cancer. Orthologous to human RAD51 (RAD51 recombinase). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 99D3-99D3; 3-100 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (30054696..30056037, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (25880418..25881759, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

we show that fork progression is enhanced in the absence of both Drosophila Rad51 homologs, spindle-A and spindle-B, revealing homologous recombination is active and actually impairs fork movement during follicle cell rereplication
Title: Multiple mechanisms contribute to double-strand break repair at rereplication forks in Drosophila follicle cells.
Data show that when both spnA and lig4 were mutated and a circular donor was provided, the frequency of ry mutations was still high and no HR products were recovered.
Title: Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila.
Ectopically expressed DmRad51 disrupts cell cycle regulation and induces apoptosis.
Title: Overexpression of Drosophila Rad51 protein (DmRad51) disrupts cell cycle progression and leads to apoptosis.
DmRad51/spnA null mutants are viable but oogenesis is disrupted by the activation of a meiotic recombination checkpoint.
Title: An essential role of DmRad51/SpnA in DNA repair and meiotic checkpoint control.
A conserved N-terminal motif in Rad54 is important for chromatin remodeling and homologous strand pairing. Its autoinhibitory activity is relieved by Rad51.
Title: A conserved N-terminal motif in Rad54 is important for chromatin remodeling and homologous strand pairing.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent DNA damage sensor activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IBA Inferred from Biological aspect of Ancestor more info
enables DNA strand exchange activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA strand exchange activity	IEA Inferred from Electronic Annotation more info
enables DNA strand exchange activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA recombinase assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA recombination	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA repair	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in DNA strand invasion	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization involved in meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via synthesis-dependent strand annealing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte fate determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	TAS Traceable Author Statement more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic recombination-dependent replication fork processing	IEA Inferred from Electronic Annotation more info
involved_in oocyte fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte karyosome formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in polarity specification of anterior/posterior axis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polarity specification of dorsal/ventral axis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in condensed nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: spindle A

Names: CG7948-PA; CG7948-PB; Rad51-like; Spindle-A; spn-A-PA; spn-A-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

30054696..30056037 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079844.4 → NP_524583.1 spindle A, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524583.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q27297, Q8IGG8, Q8IMJ5, Q9VAA8

UniProtKB/TrEMBL

C6SV45

Related

FBpp0084955

Conserved Domains (3) summary

cd01123
Location:99 → 331

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...

TIGR02239
Location:22 → 335

recomb_RAD51; DNA repair protein RAD51

pfam14520
Location:30 → 77

HHH_5; Helix-hairpin-helix domain
NM_170463.3 → NP_733342.1 spindle A, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_733342.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q27297

Related

FBpp0084956

Conserved Domains (2) summary

cd01123
Location:42 → 274

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...

pfam08423
Location:24 → 277

Rad51; Rad51