RPN2 proteasome regulatory particle base subunit RPN2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPN2
Official Full Name: proteasome regulatory particle base subunit RPN2
Primary source: SGD:S000001337
Locus tag: YIL075C
See related: AllianceGenome:SGD:S000001337; FungiDB:YIL075C; VEuPathDB:YIL075C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SEN3
Summary: Enables ubiquitin protein ligase binding activity. Contributes to endopeptidase activity. Involved in proteasome assembly and ubiquitin-dependent protein catabolic process. Located in nucleus and proteasome storage granule. Part of proteasome regulatory particle, base subcomplex. Orthologous to human PSMD1 (proteasome 26S subunit, non-ATPase 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RPN2 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (217863..220700, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
Title: Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
Comparison of the full-length Rpn1 and Rpn2 with the truncated proteins showed molecules similar in size and shape, indicating that their terminal regions are flexible and thus smeared to invisibility in the averaged images.
Title: Electron microscopic evidence in support of alpha-solenoid models of proteasomal subunits Rpn1 and Rpn2.
Rpn2 interfaces with the 20S, whereas Rpn1 sits atop Rpn2, serving as a docking site for a substrate-recruitment factor. The 19S ATPases encircle the Rpn1-Rpn2 stack, covering the remainder of the 20S surface.
Title: The central unit within the 19S regulatory particle of the proteasome.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables enzyme regulator activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in proteasome assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle, base subcomplex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome regulatory particle, base subcomplex	IDA Inferred from Direct Assay more info	PubMed
is_active_in proteasome storage granule	IBA Inferred from Biological aspect of Ancestor more info
located_in proteasome storage granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle base subunit RPN2

NP_012190.1

Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

217863..220700 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179425.1 → NP_012190.1 TPA: proteasome regulatory particle base subunit RPN2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012190.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVK9, P32565

UniProtKB/TrEMBL

B3LTS6, C8ZAH0, N1P4H3

Conserved Domains (2) summary

COG5116
Location:1 → 945

RPN2; 26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones]

sd00044
Location:533 → 557

HEAT; HEAT repeat [structural motif]