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    Akap4 A kinase anchor protein 4 [ Mus musculus (house mouse) ]

    Gene ID: 11643, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Akap4provided by MGI
    Official Full Name
    A kinase anchor protein 4provided by MGI
    Primary source
    MGI:MGI:102794
    See related
    Ensembl:ENSMUSG00000050089 AllianceGenome:MGI:102794
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    p82; Fsc1; PRKA4; AKAP-4; AKAP82; mAKAP82
    Summary
    Enables protein kinase A binding activity. Involved in flagellated sperm motility and spermatogenesis. Acts upstream of or within several processes, including cell surface receptor protein serine/threonine kinase signaling pathway; establishment of protein localization; and sperm flagellum assembly. Located in Z disc; perinuclear region of cytoplasm; and sperm flagellum. Orthologous to human AKAP4 (A-kinase anchoring protein 4). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Restricted expression toward testis adult (RPKM 214.6) See more
    Orthologs
    human all
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    Genomic context

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    See Akap4 in Genome Data Viewer
    Location:
    X A1.1; X 3.17 cM
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (6933754..6944848)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (7067515..7078609)

    Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene diacylglycerol kinase kappa Neighboring gene cyclin B3 Neighboring gene STARR-seq mESC enhancer starr_46659 Neighboring gene STARR-seq mESC enhancer starr_46660 Neighboring gene ribosomal protein L23a pseudogene Neighboring gene chloride channel, voltage-sensitive 5 Neighboring gene predicted gene, 26401

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath component. Fulcher KD, et al. Biol Reprod, 1995 Jan. PMID 7711182
    2. Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS. Choowong-In P, et al. Andrologia, 2021 Apr. PMID 33486757
    3. Protein domains govern the intracellular distribution of mouse sperm AKAP4. Nipper RW, et al. Biol Reprod, 2006 Aug. PMID 16687648
    4. Proteomics and single-cell RNA analysis of Akap4-knockout mice model confirm indispensable role of Akap4 in spermatogenesis. Fang X, et al. Dev Biol, 2019 Oct 15. PMID 31255637
    5. Tracking human multiple myeloma xenografts in NOD-Rag-1/IL-2 receptor gamma chain-null mice with the novel biomarker AKAP-4. Mirandola L, et al. BMC Cancer, 2011 Sep 16. PMID 21923911, Free PMC Article

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Identification of CD8(+) T-cell epitope from multiple myeloma-specific antigen AKAP4.
      Title: Identification of CD8(+) T-cell epitope from multiple myeloma-specific antigen AKAP4.
    2. Loss-of-function missense variant of AKAP4 induced male infertility through reduced interaction with QRICH2 during sperm flagella development.
      Title: Loss-of-function missense variant of AKAP4 induced male infertility through reduced interaction with QRICH2 during sperm flagella development.
    3. Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS.
      Title: Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS.
    4. Phosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation.
      Title: Phosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation.
    5. we validated the use of AKAP-4 antigen to track tumor growth in vivo and to specifically identify MM cells in mouse tissues.
      Title: Tracking human multiple myeloma xenografts in NOD-Rag-1/IL-2 receptor gamma chain-null mice with the novel biomarker AKAP-4.
    6. AKAP4 has a major role in completing fibrous sheath assembly
      Title: A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum.
    7. Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility.
      Title: Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility.
    8. the distribution of AKAP4 was dependent on the microtubular cytoskeleton.
      Title: Protein domains govern the intracellular distribution of mouse sperm AKAP4.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein kinase A binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein kinase A binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cell surface receptor protein serine/threonine kinase signaling pathway IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within establishment of localization in cell IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within establishment of protein localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in flagellated sperm motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in flagellated sperm motility ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within sperm flagellum assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in spermatogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Z disc IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cilium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in motile cilium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in motile cilium ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in sperm end piece IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in sperm end piece ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in sperm fibrous sheath IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in sperm fibrous sheath IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in sperm head-tail coupling apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in sperm head-tail coupling apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in sperm midpiece IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in sperm midpiece ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in sperm principal piece IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in sperm principal piece IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in sperm principal piece ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    A-kinase anchor protein 4
    Names
    A kinase (PRKA) anchor protein 4
    A kinase anchoring protein 4
    A-kinase anchor protein 82 kDa
    major sperm fibrous sheath protein
    protein kinase A-anchoring protein 4

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001042542.2 → NP_001036007.1  A-kinase anchor protein 4 isoform b

      See identical proteins and their annotated locations for NP_001036007.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a.
      Source sequence(s)
      AL808124
      Consensus CDS
      CCDS40839.1
      UniProtKB/TrEMBL
      Q9Z263
      Related
      ENSMUSP00000111417.2, ENSMUST00000115751.3
      Conserved Domains (1) summary
      cl14906
      Location:5 → 840
      AKAP_110; A-kinase anchor protein 110 kDa (AKAP 110)

    2. NM_009651.4 → NP_033781.2  A-kinase anchor protein 4 isoform a

      See identical proteins and their annotated locations for NP_033781.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longer isoform (a).
      Source sequence(s)
      AL808124, BC130271
      Consensus CDS
      CCDS40838.1
      UniProtKB/Swiss-Prot
      B1AXM7, Q60630, Q60662, Q8R2G7
      UniProtKB/TrEMBL
      A1L3T6, Q9Z263
      Related
      ENSMUSP00000050962.7, ENSMUST00000057101.13
      Conserved Domains (1) summary
      cl14906
      Location:14 → 849
      AKAP_110; A-kinase anchor protein 110 kDa (AKAP 110)

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000086.8 Reference GRCm39 C57BL/6J

      Range
      6933754..6944848
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q60662.1 GenPept UniProtKB/Swiss-Prot:Q60662

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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