RRM3 DNA helicase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RRM3
Official Full Name: DNA helicase
Primary source: SGD:S000001073
Locus tag: YHR031C
See related: AllianceGenome:SGD:S000001073; FungiDB:YHR031C; VEuPathDB:YHR031C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RTT104
Summary: Enables DNA helicase activity and centromeric DNA binding activity. Involved in several processes, including G-quadruplex DNA unwinding; replication fork progression beyond termination site; and replication fork reversal. Located in nuclear replication fork. Orthologous to human PIF1 (PIF1 5'-to-3' DNA helicase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RRM3 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (170799..172970, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The increase in cell death rates in caloric restricted cells of the yeast helicase mutant rrm3 is Sir complex dependent.
Title: The increase in cell death rates in caloric restricted cells of the yeast helicase mutant rrm3 is Sir complex dependent.
Sen1 and Rrm3 ensure permissive topological conditions for replication termination.
Title: Sen1 and Rrm3 ensure permissive topological conditions for replication termination.
Helicase activities of Rad5 and Rrm3 genetically interact in the prevention of recombinogenic DNA lesions in Saccharomyces cerevisiae.
Title: Helicase activities of Rad5 and Rrm3 genetically interact in the prevention of recombinogenic DNA lesions in Saccharomyces cerevisiae.
Pif1 family helicases promote mutation avoidance during DNA replication.
Title: Pif1 family helicases promote mutation avoidance during DNA replication.
Suppression of chromosome instability by targeting a DNA helicase in budding yeast.
Title: Suppression of chromosome instability by targeting a DNA helicase in budding yeast.
We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo.
Title: Pif1-Family Helicases Support Fork Convergence during DNA Replication Termination in Eukaryotes.
The absence of Rrm3 renders cells resistant to microtubule-destabilizing drugs.
Title: Two Pif1 Family DNA Helicases Cooperate in Centromere Replication and Segregation in Saccharomyces cerevisiae.
It has been proposed that Rrm3 and Pif1 promote genome stability at tDNAs by displacing the stable multi-protein transcription complex and by removing R-loops.
Title: PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes.
Either Pif1 or Rrm3 redundantly stimulates strand displacement by DNA polymerase delta during lagging-strand synthesis.
Title: Pif1-family helicases cooperatively suppress widespread replication-fork arrest at tRNA genes.
Rrm3 recruitment to replication-born DSBs is crucial for viability.
Title: A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-3' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-3' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables G-quadruplex DNA binding	IEA Inferred from Electronic Annotation more info
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G-quadruplex DNA unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in G-quadruplex DNA unwinding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial genome maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial genome maintenance	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial genome maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in replication fork progression beyond termination site	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork reversal	IBA Inferred from Biological aspect of Ancestor more info
involved_in replication fork reversal	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nuclear replication fork	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in replication fork	IEA Inferred from Electronic Annotation more info
located_in replication fork	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: DNA helicase

NP_011896.1

EC 3.6.4.12

DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; deletion or inactivation of helicase function suppresses chromosome instability (CIN) associated with centromere or kinetochore defects; structurally and functionally related to Pif1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

170799..172970 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179161.1 → NP_011896.1 TPA: DNA helicase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011896.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKX8, P38766

UniProtKB/TrEMBL

N1P392

Conserved Domains (2) summary

COG0507
Location:37 → 723

RecD; ATP-dependent exoDNAse (exonuclease V), alpha subunit, helicase superfamily I [Replication, recombination and repair]

cd18037
Location:235 → 414

DEXSc_Pif1_like; DEAD-box helicase domain of Pif1