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    putP proline:Na(+) symporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945602, updated on 3-Dec-2024

    Summary

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    Official Symbol
    putP
    Official Full Name
    proline:Na(+) symporter
    Primary source
    ECOCYC:EG10802
    Locus tag
    b1015
    See related
    ASAP:ABE-0003433
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1006; putC
    Summary
    PutP is a member of the SSS family of sodium/solute transporters . [More information is available at EcoCyc: EG10802].
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    Genomic context

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    Sequence:
    NC_000913.3 (1079305..1080813)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene fused DNA-binding transcriptional repressor/proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase PutA Neighboring gene protein YmdG Neighboring gene pseudo Neighboring gene ferrous iron transport system protein EfeO

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli. Yamato I, et al. J Biol Chem, 1994 Feb 25. PMID 8119910
    2. Charge translocation during cosubstrate binding in the Na+/proline transporter of E.coli. Zhou A, et al. J Mol Biol, 2004 Oct 29. PMID 15476811
    3. Interresidual distance determination by four-pulse double electron-electron resonance in an integral membrane protein: the Na+/proline transporter PutP of Escherichia coli. Jeschke G, et al. Biophys J, 2004 Apr. PMID 15041691, Free PMC Article
    4. Sites important for Na+ and substrate binding in the Na+/proline transporter of Escherichia coli, a member of the Na+/solute symporter family. Pirch T, et al. J Biol Chem, 2002 Mar 15. PMID 11756453
    5. Core Transmembrane Domain 6 Plays a Pivotal Role in the Transport Cycle of the Sodium/Proline Symporter PutP. Bracher S, et al. J Biol Chem, 2016 Dec 9. PMID 27793991, Free PMC Article

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. the results demonstrate that TM6' plays a central role in substrate binding and release on the inner side of the membrane also in PutP and extend the knowledge on functionally relevant amino acids in transporters with a LeuT-type structural fold.
      Title: Core Transmembrane Domain 6 Plays a Pivotal Role in the Transport Cycle of the Sodium/Proline Symporter PutP.
    2. Remarkably,as proposed computationally and discovered here experimentally, residues Y140, W244, and Y248 of transmembrane segments 4 and 7 are particularly important for PutP function and are suggested to participate in proline binding and/or gating.
      Title: Homology model of the Na+/proline transporter PutP of Escherichia coli and its functional implications.
    3. backbone models of PutP exhibiting a pronounced kink near residue T341, which is involved in substrate binding, were obtained. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity.
      Title: Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli.
    4. Results describe the function of transmembrane domain IX in the Na+/proline transporter PutP.
      Title: Function of transmembrane domain IX in the Na+/proline transporter PutP.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Sodium ion and proline binding sites in the Na+/proline symport carrier of Escherichia coli.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables L-proline transmembrane transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables proline:sodium symporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables proline:sodium symporter activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables proline:sodium symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sodium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables symporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in amino acid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carboxylic acid transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carboxylic acid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proline transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proline transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within proline transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proline transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within proline transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within short-chain fatty acid transport IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within short-chain fatty acid transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sodium ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    proline:Na(+) symporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1079305..1080813
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415535.1 proline:Na(+) symporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415535.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376YAE2
      Conserved Domains (1) summary
      PRK15419
      Location:1 → 502
      PRK15419; sodium/proline symporter PutP
    Nucleotide Protein
    Heading Accession and Version

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