U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    SPB1 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850304, updated on 9-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    SPB1
    Official Full Name
    27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
    Primary source
    SGD:S000000559
    Locus tag
    YCL054W
    See related
    AllianceGenome:SGD:S000000559; FungiDB:YCL054W; VEuPathDB:YCL054W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables rRNA (guanine) methyltransferase activity and rRNA (uridine-2'-O-)-methyltransferase activity. Involved in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); and rRNA methylation. Located in nucleolus. Part of preribosome, large subunit precursor. Orthologous to human FTSJ3 (FtsJ RNA 2'-O-methyltransferase 3). [provided by Alliance of Genome Resources, Dec 2024]
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See SPB1 in Genome Data Viewer
    Location:
    chromosome: III
    Exon count:
    1
    Sequence:
    Chromosome: III; NC_001135.5 (31449..33974)

    Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene Kar4p Neighboring gene Rdt1p Neighboring gene Pbn1p Neighboring gene Lre1p

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_001135.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Kressler D, et al. Nucleic Acids Res, 1999 Dec 1. PMID 10556316, Free PMC Article
    2. Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage. Lapeyre B, et al. Mol Cell, 2004 Nov 19. PMID 15546625
    3. Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast. Bonnerot C, et al. Mol Cell, 2003 Nov. PMID 14636587
    4. Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop. Pintard L, et al. EMBO J, 2002 Apr 2. PMID 11927565, Free PMC Article
    5. Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner. Brandariz-Núñez A, et al. RNA, 2018 Jan. PMID 28986506, Free PMC Article

    See all (86) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA (guanine) methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA (guanine) methyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables rRNA (guanosine-2'-O-)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA (uridine-2'-O-)-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables rRNA (uridine-2'-O-)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rRNA (uridine-2'-O-)-methyltransferase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables rRNA methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA 2'-O-methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA methylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in rRNA methylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in rRNA methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in rRNA methylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in rRNA methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of preribosome, large subunit precursor IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of preribosome, large subunit precursor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of preribosome, large subunit precursor IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
    NP_009877.1
    • AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001135.5 Reference assembly

      Range
      31449..33974
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178698.1 → NP_009877.1  TPA: 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009877.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQW3, P25582
      UniProtKB/TrEMBL
      A6ZTE1, C7GT08, G2W9X4, N1PAN5
      Conserved Domains (3) summary
      COG0293
      Location:1 → 206
      RlmE; 23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and biogenesis]
      pfam07780
      Location:621 → 835
      Spb1_C; Spb1 C-terminal domain
      pfam11861
      Location:236 → 383
      DUF3381; Domain of unknown function (DUF3381)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25582.2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools