STT3 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STT3
Official Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Primary source: SGD:S000002990
Locus tag: YGL022W
See related: AllianceGenome:SGD:S000002990; FungiDB:YGL022W; VEuPathDB:YGL022W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Predicted to enable dolichyl-diphosphooligosaccharide-protein glycotransferase activity. Involved in protein N-linked glycosylation. Located in endoplasmic reticulum. Part of oligosaccharyltransferase complex. Used to study congenital disorder of glycosylation Iw. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Iw and congenital disorder of glycosylation Ix. Orthologous to several human genes including STT3B (STT3 oligosaccharyltransferase complex catalytic subunit B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See STT3 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (452404..454560)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyl transfer reaction by point mutations in yeast oligosaccharyltransferase.
Title: Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyl transfer reaction by point mutations in yeast oligosaccharyltransferase.
results show that most of the STT3 TMDs are well inserted into the ER membrane on their own or in the presence of the natural flanking residues.
Title: Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyltransferase complex.
Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase.
Title: Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase.
The cloning, overexpression, purification, and biophysical characterization of the carboxyl-terminal domain of Stt3p is reported; Asp(518) plays a critical structural role, in addition to its previously proposed catalytic role.
Title: A novel method of production and biophysical characterization of the catalytic domain of yeast oligosaccharyl transferase.
is an essential player in N-glycosylation
Title: Unraveling the mechanism of protein N-glycosylation.
results suggest that membrane protein STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation
Title: Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex.
Homologous Stt3 proteins can serve analogous functions in distantly related eukaryotic cells regardless of their degree of conservation.
Title: High-level expression of the Toxoplasma gondii STT3 gene is required for suppression of the yeast STT3 gene mutation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity	IC Inferred by Curator more info	PubMed
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity	IEA Inferred from Electronic Annotation more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oligosaccharyl transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in post-translational protein modification	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein N-linked glycosylation	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein N-linked glycosylation via asparagine	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein glycosylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of oligosaccharyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
part_of oligosaccharyltransferase complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

NP_011493.1

EC 2.4.99.18

Catalytic subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER lumen; involved in recognition of N-acetylglucosamine; forms a subcomplex with Ost3p and Ost4p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

452404..454560

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180887.1 → NP_011493.1 TPA: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011493.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUB5, P39007

UniProtKB/TrEMBL

A6ZUX1, B5VIT9, C7GSA2, G2WE71, N1P3P8

Conserved Domains (1) summary

pfam02516
Location:15 → 475

STT3; Oligosaccharyl transferase STT3 subunit