pins partner of inscuteable [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pinsprovided by FlyBase
Official Full Name: partner of inscuteableprovided by FlyBase
Primary source: FLYBASE:FBgn0040080
Locus tag: Dmel_CG5692
See related: AllianceGenome:FB:FBgn0040080
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG5692; dLGN; Dmel\CG5692; dPins; LGN; Pins; PINS; pins/raps; rad; Rad; raps; Raps; RAPS; rapsyn
Summary: Enables GDP-dissociation inhibitor activity and protein domain specific binding activity. Involved in asymmetric neuroblast division and establishment of mitotic spindle orientation. Located in apical cortex. Part of raps-insc complex. Is expressed in several structures, including embryonic/larval midgut; embryonic/larval midgut primordium; epithelium; larval muscle system; and neuroblasts. Used to study brain cancer and cancer. Human ortholog(s) of this gene implicated in sensorineural hearing loss. Orthologous to human GPSM1 (G protein signaling modulator 1) and GPSM2 (G protein signaling modulator 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 98A8-98A8; 3-94 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (27582840..27586376)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (23408562..23412098)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pins is not required in the wing disc because there are parallel mechanisms for Mud localization and hence spindle orientation, making it a more robust system than in other epithelia.
Title: Pins is not required for spindle orientation in the Drosophila wing disc.
Prefoldin acts synergistically with Pins to regulate asymmetric division of both neuroblasts and Intermediate Neural Progenitors.
Title: Prefoldin and Pins synergistically regulate asymmetric division and suppress dedifferentiation.
The sterical hindrance requiring such a rotation probably contributes to the guanine nucleotide exchange inhibitor activity of GoLoco1 and Pins as a whole.
Title: Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine nucleotides.
Insc-Pins complex requires Galphai for partial activity and that the complex specifically recruits Dlg but not Mud.
Title: Inscuteable regulates the Pins-Mud spindle orientation pathway.
The Canoe-Pins(TPR) interaction recruits Canoe to the cell cortex and is required for activation of the Pins(TPR)-Mud (nuclear mitotic apparatus in mammals) spindle orientation pathway
Title: Canoe binds RanGTP to promote Pins(TPR)/Mud-mediated spindle orientation.
Results identify Drosophila Pins as a target of Galpha(o)-mediated GPCR receptor signaling, where Galpha(o) acts downstream from Frizzled and redundantly with Galpha(i) to control the asymmetry of cell divisions.
Title: Drosophila GoLoco-protein Pins is a target of Galpha(o)-mediated G protein-coupled receptor signaling.
Study identified a previously unrecognized evolutionarily conserved Pins domain (Pins(LINKER)) that requires Aurora-A phosphorylation to recruit Dlg and promote partial spindle orientation.
Title: Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells.
Pins forms a "closed" state via intramolecular GoLoco-tetratricopeptide repeat (TPR) interactions, which regulate Mud binding
Title: Galphai generates multiple Pins activation states to link cortical polarity and spindle orientation in Drosophila neuroblasts.
Stbm-dependent recruitment of Pins protein at the anterior cortex of the sense organ precursor cell is a novel read-out of planar cell polarity.
Title: The planar cell polarity protein Strabismus promotes Pins anterior localization during asymmetric division of sensory organ precursor cells in Drosophila.
regulated formation of a protein complex consisting of LKB1, AGS3, and G-protein, and phosphorylation of AGS3 GPR domains by LKB1, regulating the interaction of GPR-containing proteins with G-proteins
Title: Interaction of activator of G-protein signaling 3 (AGS3) with LKB1, a serine/threonine kinase involved in cell polarity and cell cycle progression: phosphorylation of the G-protein regulatory (GPR) motif as a regulatory mechanism for the interaction of GPR motifs with Gi alpha.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables G-protein alpha-subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables GDP-dissociation inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables GDP-dissociation inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase regulator activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in asymmetric cell division	IDA Inferred from Direct Assay more info	PubMed
involved_in asymmetric cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric neuroblast division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	TAS Traceable Author Statement more info	PubMed
involved_in basal protein localization	TAS Traceable Author Statement more info	PubMed
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of mitotic spindle orientation	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
part_of raps-insc complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: partner of inscuteable

Names: CG5692-PA; partner of inscutable; pins-PA; rapsinoid; rapsynoid

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

27582840..27586376

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080260.4 → NP_524999.2 partner of inscuteable [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524999.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9NH88, Q9VB22

Related

FBpp0084559

Conserved Domains (4) summary

smart00028
Location:219 → 251

TPR; Tetratricopeptide repeats

sd00006
Location:219 → 246

TPR; TPR repeat [structural motif]

pfam02188
Location:468 → 488

GoLoco; GoLoco motif

pfam13424
Location:78 → 153

TPR_12; Tetratricopeptide repeat