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    NPR2 nitrogen permease regulating protein NPR2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856647, updated on 9-Dec-2024

    Summary

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    Official Symbol
    NPR2
    Official Full Name
    nitrogen permease regulating protein NPR2
    Primary source
    SGD:S000000788
    Locus tag
    YEL062W
    See related
    AllianceGenome:SGD:S000000788; FungiDB:YEL062W; VEuPathDB:YEL062W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Predicted to contribute to GTPase activator activity. Involved in several processes, including cellular response to amino acid starvation; negative regulation of TORC1 signaling; and proline transport. Part of GATOR1 complex. Used to study cancer. Human ortholog(s) of this gene implicated in familial focal epilepsy with variable foci 2. Orthologous to human NPRL2 (NPR2 like, GATOR1 complex subunit). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See NPR2 in Genome Data Viewer
    Location:
    chromosome: V
    Exon count:
    1
    Sequence:
    Chromosome: V; NC_001137.3 (34407..36254)

    Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene Avt2p Neighboring gene arginine permease CAN1 Neighboring gene kinesin motor protein CIN8 Neighboring gene proteinase B

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001137.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A second nitrogen permease regulator in Saccharomyces cerevisiae. Rousselet G, et al. FEBS Lett, 1995 Feb 13. PMID 7867803
    2. Anticancer drug resistance induced by disruption of the Saccharomyces cerevisiae NPR2 gene: a novel component involved in cisplatin- and doxorubicin-provoked cell kill. Schenk PW, et al. Mol Pharmacol, 2003 Aug. PMID 12869630
    3. The SEACIT complex is involved in the maintenance of vacuole-mitochondria contact sites and controls mitophagy. Ma Y, et al. Cell Mol Life Sci, 2019 Apr. PMID 30673821, Free PMC Article
    4. Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Wu X, et al. Mol Biol Cell, 2011 Nov. PMID 21900499, Free PMC Article
    5. GATOR1 regulates nitrogenic cataplerotic reactions of the mitochondrial TCA cycle. Chen J, et al. Nat Chem Biol, 2017 Nov. PMID 28920930, Free PMC Article

    See all (72) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. These data revealed the metabolic basis by which the Npr2 complex regulates cellular homeostasis and demonstrated a key function for TORC1 in regulating the synthesis and utilization of glutamine as a nitrogen source.
      Title: Npr2 inhibits TORC1 to prevent inappropriate utilization of glutamine for biosynthesis of nitrogen-containing metabolites.
    2. Npr2-Npr3-dependent GTP hydrolysis of Gtr1 and direct binding of Gtr2 to Kog1, are involved in TORC1 inactivation and autophagic induction
      Title: Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy.
    3. A visual screen uncovered three regulators of autophagy-Iml1p, Npr2p, and Npr3p-which function in the same complex and are selectively required for non-nitrogen starvation-induced autophagy
      Title: Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation.
    4. Data indicate that Npr2, a homolog of human NPRL2, is a phosphorylation-dependent target of the SCF(Grr1) E3 ubiquitin ligase that plays a role in cell growth on some nitrogen sources.
      Title: Npr2, yeast homolog of the human tumor suppressor NPRL2, is a target of Grr1 required for adaptation to growth on diverse nitrogen sources.
    5. Npr2/3 complex mediates an amino acid starvation signal to TORC1.
      Title: A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    contributes_to GTPase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular response to amino acid starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to nitrogen starvation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in cellular response to nitrogen starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of TORC1 signaling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of TORC1 signaling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of TORC1 signaling IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of TORC1 signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of autophagy IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of autophagy IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of autophagy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proline transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of autophagosome assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in response to xenobiotic stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in urea transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of GATOR1 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of GATOR1 complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of Seh1-associated complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in vacuolar membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in vacuolar membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    nitrogen permease regulating protein NPR2
    NP_010852.4
    • Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001137.3 Reference assembly

      Range
      34407..36254
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178877.3 → NP_010852.4  TPA: nitrogen permease regulating protein NPR2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010852.4

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D3DLI8, P39923, Q6B264
      UniProtKB/TrEMBL
      A6ZQN9, G2WCH2
      Conserved Domains (1) summary
      cl24021
      Location:9 → 490
      NPR3; Nitrogen Permease regulator of amino acid transport activity 3
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P39923.2

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