U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    lptA lipopolysaccharide transport system protein LptA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947920, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    lptA
    Official Full Name
    lipopolysaccharide transport system protein LptA
    Primary source
    ECOCYC:G7665
    Locus tag
    b3200
    See related
    ASAP:ABE-0010510
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3189; yhbN
    Summary
    LptA depletion results in rifampicin/bile salts sensitivity, indicative of an outer membrane integrity defect. [More information is available at EcoGene: EG12618]. LptA is a periplasmic binding component of the LptABC ABC transporter. [More information is available at EcoCyc: G7665].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (3343380..3343937)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene D-arabinose 5-phosphate isomerase KdsD Neighboring gene 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC Neighboring gene lipopolysaccharide transport system protein LptC Neighboring gene lipopolysaccharide transport system ATP binding protein LptB Neighboring gene RNA polymerase sigma factor RpoN

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli. Falchi FA, et al. J Bacteriol, 2018 Jan 15. PMID 29109183, Free PMC Article
    2. Lipopolysaccharide binding to the periplasmic protein LptA. Schultz KM, et al. Protein Sci, 2017 Aug. PMID 28419595, Free PMC Article
    3. An induced folding process characterizes the partial-loss of function mutant LptAI36D in its interactions with ligands. Santambrogio C, et al. Biochim Biophys Acta, 2015 Oct. PMID 26123264
    4. The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. Tran AX, et al. J Biol Chem, 2008 Jul 18. PMID 18480051, Free PMC Article
    5. LptA assembles into rod-like oligomers involving disorder-to-order transitions. Santambrogio C, et al. J Am Soc Mass Spectrom, 2013 Oct. PMID 23897621

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Characterized interactions and binding of lipopolysaccharide transport system proteins LptA and LptC thru dimerization and point mutation studies.
      Title: Disruption of the E. coli LptC dimerization interface and characterization of lipopolysaccharide and LptA binding to monomeric LptC.
    2. characterization of a viable quadruple lptA mutant impaired in the assembly of the Lpt machinery, although it retains the ability to bind LPS; although viable, the quadruple lptA mutant disrupts outer membrane (OM) asymmetry and severely impairs the OM permeability barrier; analysis of 2 independent suppressors revealed different strategies adopted by the cell to overcome defects in LPS biogenesis
      Title: Mutation and Suppressor Analysis of the Essential Lipopolysaccharide Transport Protein LptA Reveals Strategies To Overcome Severe Outer Membrane Permeability Defects in Escherichia coli.
    3. LptA molecular structure is affected by lipopolysaccharides binding.
      Title: Lipopolysaccharide binding to the periplasmic protein LptA.
    4. An induced folding process characterizes the partial-loss of function mutant LptAI36D in its interactions with lipopolysaccharide constituent ligands.
      Title: An induced folding process characterizes the partial-loss of function mutant LptAI36D in its interactions with ligands.
    5. Studied the assembly and architecture of LptA oligomers.
      Title: LptA assembles into rod-like oligomers involving disorder-to-order transitions.
    6. results suggest that LptA operate in the LPS assembly pathway and, together with other as-yet-unidentified components, could be part of a complex devoted to the transport of LPS from the periplasmic surface of the IM to the OM.
      Title: Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli.
    7. LptA binds structurally diverse lipopolysaccharide (LPS) substrates in vitro and demonstrate that it interacts specifically with the lipid A domain of LPS
      Title: The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway.
    8. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables glycolipid transfer activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glycolipid transfer activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipopolysaccharide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Gram-negative-bacterium-type cell outer membrane assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glycolipid transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipopolysaccharide transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in lipopolysaccharide transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipopolysaccharide transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cell outer membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in outer membrane-bounded periplasmic space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of transporter complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    lipopolysaccharide transport system protein LptA
    NP_417667.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3343380..3343937
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417667.1 lipopolysaccharide transport system protein LptA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417667.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9APM3, A0A5D8MTI4
      Conserved Domains (1) summary
      PRK10894
      Location:5 → 185
      PRK10894; lipopolysaccharide ABC transporter substrate-binding protein LptA
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.