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    ushA 5'-nucleotidase/UDP-sugar hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947331, updated on 17-Dec-2024

    Summary

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    Official Symbol
    ushA
    Official Full Name
    5'-nucleotidase/UDP-sugar hydrolase
    Primary source
    ECOCYC:EG11060
    Locus tag
    b0480
    See related
    ASAP:ABE-0001668
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0474
    Summary
    UshA has highly efficient CDP-alcohol hydrolase activity (Alves-Pereira, 2008). [More information is available at EcoGene: EG11060]. Salmonella has a different gene and enzyme, ushB. [More information is available at EcoCyc: EG11060].
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    Genomic context

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    Sequence:
    NC_000913.3 (504914..506566)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative transporter YbaL Neighboring gene fosmidomycin efflux pump Neighboring gene Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK Neighboring gene ncRNA Neighboring gene TraB family protein YbaP

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Synthesis of Escherichia coli uridine diphosphate glucose hydrolase, and demonstration of an inhibitor, in Salmonella typhimurium cytoplasm. Taylor N, et al. Biochem Soc Trans, 1975. PMID 1093908
    2. The 5'-nucleotidase of Escherichia coli. II. Surface localization and purification of the Escherichia coli 5'-nucleotidase inhibitor. Neu HC. J Biol Chem, 1967 Sep 10. PMID 5341266
    3. Molecular cloning of the gene (ush) from Escherichia coli specifying periplasmic UDP-sugar hydrolase (5'-nucleotidase). Cowman A, et al. Gene, 1980 Dec. PMID 6265322
    4. Characterization of the ush gene of Escherichia coli and its protein products. Burns DM, et al. Gene, 1983 Nov. PMID 6363215
    5. Kinetic characteristics of nucleoside mono-, di- and triphosphatase activities of the periplasmic 5'-nucleotidase of Escherichia coli. García L, et al. Comp Biochem Physiol B Biochem Mol Biol, 1997 May. PMID 9180021

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. CDP-alcohol hydrolase activity was added to the spectrum of UshA enzyme activities.
      Title: CDP-alcohol hydrolase, a very efficient activity of the 5'-nucleotidase/UDP-sugar hydrolase encoded by the ushA gene of Yersinia intermedia and Escherichia coli.
    2. An ushA aphA double knockout mutant was shown to be unable to grow on purine nucleotides as the sole carbon source.
      Title: Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    4. Two variants of Escherichia coli 5'-nucleotidase with disulfide bridges that are engineered to link the two domains of the protein are used to demonstrate that a large domain rotation is required for the catalytic mechanism of the enzyme.
      Title: A large hinge bending domain rotation is necessary for the catalytic function of Escherichia coli 5'-nucleotidase.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 5'-nucleotidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 5'-nucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 5'-nucleotidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables UDP-sugar diphosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables UDP-sugar diphosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables UDP-sugar diphosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables XMP 5'-nucleosidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on ester bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within nucleotide catabolic process EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in nucleotide catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in outer membrane-bounded periplasmic space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in periplasmic space IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    5'-nucleotidase/UDP-sugar hydrolase
    NP_415013.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      504914..506566
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415013.1 5'-nucleotidase/UDP-sugar hydrolase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415013.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MSJ5
      Conserved Domains (1) summary
      PRK09558
      Location:1 → 550
      ushA; bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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