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    amiD N-acetylmuramoyl-L-alanine amidase D [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945494, updated on 3-Dec-2024

    Summary

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    Official Symbol
    amiD
    Official Full Name
    N-acetylmuramoyl-L-alanine amidase D
    Primary source
    ECOCYC:G6452
    Locus tag
    b0867
    See related
    ASAP:ABE-0002947
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0858; ybjR
    Summary
    AmiD also cleaves N-acetylmuramyl-L-alanine bonds in murein and muropeptides (Uehara, 2007). [More information is available at EcoGene: EG13687]. AmiD is an 1,6-anhydro-N-acetylmuramic acid (anhMurNAc)-L-alanine amidase responsible for cleaving the bond between muramic acid and L-alanine within murein, muropeptides, and anhydro-muropeptides. [More information is available at EcoCyc: G6452].
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    Genomic context

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    Sequence:
    NC_000913.3 (904913..905743)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene L-arginine ABC transporter ATP binding subunit Neighboring gene DUF3828 domain-containing lipoprotein YbjP Neighboring gene putative heavy metal binding protein YbjQ Neighboring gene putative epimerase YbjS Neighboring gene putative NAD(P)-binding protein YbjT Neighboring gene low-specificity L-threonine aldolase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Substrate-induced inactivation of the Escherichia coli AmiD N-acetylmuramoyl-L-alanine amidase highlights a new strategy to inhibit this class of enzyme. Pennartz A, et al. Antimicrob Agents Chemother, 2009 Jul. PMID 19237650, Free PMC Article
    2. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. Beck BD, et al. J Bacteriol, 1976 Jun. PMID 780345, Free PMC Article
    3. An anhydro-N-acetylmuramyl-L-alanine amidase with broad specificity tethered to the outer membrane of Escherichia coli. Uehara T, et al. J Bacteriol, 2007 Aug. PMID 17526703, Free PMC Article
    4. Prediction of lipoprotein signal peptides in Gram-negative bacteria. Juncker AS, et al. Protein Sci, 2003 Aug. PMID 12876315, Free PMC Article
    5. Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12. Gonnet P, et al. Proteomics, 2004 Jun. PMID 15174130

    See all (10) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. we recognized YbjR, now renamed AmiD, as a second 1,6-anhydro-N-acetylmuramic acid (anhMurNAc)-l-alanine amidase in Escherichia coli.
      Title: An anhydro-N-acetylmuramyl-L-alanine amidase with broad specificity tethered to the outer membrane of Escherichia coli.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables N-acetyl-anhydromuramoyl-L-alanine amidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N-acetylmuramoyl-L-alanine amidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables N-acetylmuramoyl-L-alanine amidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N-acetylmuramoyl-L-alanine amidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cell wall organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in peptidoglycan catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in peptidoglycan catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in peptidoglycan catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in peptidoglycan turnover IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in outer membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    N-acetylmuramoyl-L-alanine amidase D
    NP_415388.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      904913..905743
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415388.1 N-acetylmuramoyl-L-alanine amidase D [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415388.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7QYF6
      Conserved Domains (1) summary
      COG3023
      Location:16 → 272
      AmpD; N-acetyl-anhydromuramyl-L-alanine amidase AmpD [Cell wall/membrane/envelope biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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