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    recQ ATP-dependent DNA helicase RecQ [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948318, updated on 3-Dec-2024

    Summary

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    Official Symbol
    recQ
    Official Full Name
    ATP-dependent DNA helicase RecQ
    Primary source
    ECOCYC:EG10833
    Locus tag
    b3822
    See related
    ASAP:ABE-0012483
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3816
    Summary
    SSB binds to RecQ and stimulates DNA unwinding (Shereda, 2007). [More information is available at EcoGene: EG10833]. A component of the RecF recombinational pathway, RecQ is a helicase that functions in blocking illegitimate recombination, enhancing topoisomerase activity, initiating SOS signaling and clearing blocked replication forks. [More information is available at EcoCyc: EG10833].
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    Genomic context

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    Sequence:
    NC_000913.3 (4005864..4007693)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative thioesterase YigI Neighboring gene outer membrane phospholipase A Neighboring gene L-threonine exporter Neighboring gene L-homoserine/L-homoserine lactone/L-threonine exporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The DNA binding properties of the Escherichia coli RecQ helicase. Dou SX, et al. J Biol Chem, 2004 Feb 20. PMID 14665634
    2. Biochemical characterization of the DNA helicase activity of the escherichia coli RecQ helicase. Harmon FG, et al. J Biol Chem, 2001 Jan 5. PMID 11024023
    3. Inhibition of DNA helicase, ATPase and DNA-binding activities of E. coli RecQ helicase by chemotherapeutic agents. Zhang B, et al. J Biochem, 2008 Jun. PMID 18296713
    4. Substrate-specific inhibition of RecQ helicase. Wu X, et al. Nucleic Acids Res, 2001 Apr 15. PMID 11292849, Free PMC Article
    5. Function of recQ family helicase in genome stability. Seki M, et al. Subcell Biochem, 2006. PMID 17623900

    See all (78) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Inhibition of E. coli RecQ Helicase Activity by Structurally Distinct DNA Lesions: Structure-Function Relationships.
      Title: Inhibition of E. coli RecQ Helicase Activity by Structurally Distinct DNA Lesions: Structure-Function Relationships.
    2. Nanopore tweezers measurements of RecQ conformational changes reveal the energy landscape of helicase motion.
      Title: Nanopore tweezers measurements of RecQ conformational changes reveal the energy landscape of helicase motion.
    3. The rarA gene as part of an expanded RecFOR recombination pathway: Negative epistasis and synthetic lethality with ruvB, recG, and recQ.
      Title: The rarA gene as part of an expanded RecFOR recombination pathway: Negative epistasis and synthetic lethality with ruvB, recG, and recQ.
    4. The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex.
      Title: The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex.
    5. This study shows that replication fork regression, the combination of replication fork reversal leading to formation of a Holliday Junction along with exonuclease digestion, is the preferred pathway for dealing with a collapsed fork in Escherichia coli. The protein that had the greatest effect on these fork processing events was the RecQ helicase.
      Title: Replication fork collapse at a protein-DNA roadblock leads to fork reversal, promoted by the RecQ helicase.
    6. RecQ operated under 2 interconvertible kinetic modes, 'slow' and 'normal', as it unwound duplex DNA and translocated on single-stranded (ss) DNA. Abasic sites blocked RecQ unwinding. Removal of the HRDC domain eliminates only the slow mode. A RecQ variant lacking both the WH and HRDC domains retains weak helicase activity.
      Title: Single molecule kinetics uncover roles for E. coli RecQ DNA helicase domains and interaction with SSB.
    7. To illustrate the single-molecule unwinding assay and the analysis routine, we provide DNA unwinding measurements of Escherichia coli RecQ helicase under a variety of conditions (Na+, ATP, temperature, and DNA substrate geometry).
      Title: Single molecule measurements of DNA helicase activity with magnetic tweezers and t-test based step-finding analysis.
    8. Coupling of helicase and RecA-loading activity during dsDNA-end resection is crucial in avoiding the deleterious effects of a long and stabile 3' tail in E. coli.
      Title: Nucleolytic degradation of 3'-ending overhangs is essential for DNA-end resection in RecA-loading deficient recB mutants of Escherichia coli.
    9. Data show that the single-stranded DNA binding protein (SSB)-RecQ helicase (RecQ) interaction changes the binding mode of SSB, thereby allowing RecQ to gain access to ssDNA and facilitating DNA unwinding.
      Title: RecQ helicase triggers a binding mode change in the SSB-DNA complex to efficiently initiate DNA unwinding.
    10. the mechanochemical coupling of E. coli RecQ appears to be independent of HRDC-ssDNA and HRDC-RecA core interactions, which may play roles in more specialized functions of the enzyme.
      Title: The HRDC domain of E. coli RecQ helicase controls single-stranded DNA translocation and double-stranded DNA unwinding rates without affecting mechanoenzymatic coupling.
    Submit: New GeneRIF Correction See all GeneRIFs (24)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3'-5' DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-5' DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3'-5' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on DNA IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables forked DNA-dependent helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables four-way junction helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables four-way junction helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded 3'-5' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transition metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in DNA duplex unwinding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within DNA recombination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in G-quadruplex DNA unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in SOS response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in bacterial nucleoid IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in bacterial nucleoid IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in chromosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of replisome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of replisome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of single-stranded DNA-dependent ATP-dependent DNA helicase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ATP-dependent DNA helicase RecQ

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4005864..4007693
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. YP_026263.3 ATP-dependent DNA helicase RecQ [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for YP_026263.3

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6C9H3W5, A0A828PFW8
      Conserved Domains (1) summary
      PRK11057
      Location:1 → 607
      PRK11057; ATP-dependent DNA helicase RecQ; Provisional
    Nucleotide Protein
    Heading Accession and Version

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